组织工程软骨细胞体外培养技术要点

In vitro techniques of chondrocyte culture in tissue engineering

通过组织工程技术,采用自身软骨细胞体外增殖构建软骨来修复关节软骨损伤,在临床上已成为一种有效的新方法。近年来,有关体外软骨细胞培养技术的研究较多。通过对入选文献进行分析、整理,对影响体外构建软骨的因素包括培养空间、应力、细胞密度和氧张力等方面进行分析,体外采用三维多条件联合培养软骨细胞可以改善构建软骨的生物品质。但是影响软骨细胞生长的因素众多复杂,在体外如何更好地构建软骨,有待进一步研究。

It has been a novel clinical way to repair damaged articular cartilage with autologous chondrocytes cultured in vitro using cartilage tissue engineering technique. There have been many articles about the techniques of chondrocytes culture in recent years. After summarizing and analyzing the involved articles, factors on chondrocytes cultured in vitro were reviewed, including dimension, stress, cell density and oxygen tension, etc. Chondrocytes cultured in three-dimensional with multi-factors in vitro can promote quality and quantity of cartilage in tissue engineering. But factors on chondrocytes culture are so complicated that further researches will be needed.