α1肾上腺素受体1A亚型基因多态性与高血压患者抗α1A肾上腺受体抗体间的关联分析

Association between ADRA1A gene polymorphism and autoantibodies against the α1-adrenergic receptor in hypertensive patients

目的研究α1肾上腺素受体1A亚型基因（ADRA1A）多态性与原发性高血压患者抗α1A受体自身抗体产生间的关联性。方法随机抽取396例原发性高血压住院患者血标本并离心，采用ELISA法对血清进行抗α1A受体抗体检测并将其分为抗体阳性和阴性两组；同时，用试剂盒提取血细胞基因组DNA。运用连接酶检测反应（ligase detection reaction，LDR）检测基因ADRA1A中rs574584、rs1048101、rs3739216及rs3802241四个点的单核苷酸多态性，并进行基因表型频率统计和单倍型分析。结果匹配种族和协调变量后的抗体阴阳性组中，上述各点的基因型频率间差异无统计学意义，而行单倍型分析后得到[G-C-C—A]和[G—C—G—A]单倍型其发生频率在两组间具有明显差异，其P值分别是0．010和0．035。另外，对高血压2级患者进行抗体阴阳性分组统计得到：两组间点rs1048101（基因表型C／C、C／T，P=0．017）及rs3802241（基因表型A／A、A／G，P=0．004）基因型频率存在明显差异。结论α1A肾上腺素受体基因ADRA1A多态性与原发性高血压患者血清中α1A自身抗体产生有一定的关联性。

Objective To observe the association between ADRA1A gene polymorphism arid autoantibodies against the α1-adrenergic receptor in hypertensive patients. Methods A total of 396 patients with essential hypertension admitted to our hospital were selected and autoantibodies in sera were detected by ELISA, and patients were divided into the autoantibody positive and negative group. Genomic DNA was extracted from erythrocytes obtained from EDTA-treated blood by the Blood DNA extraction kit. Gene polymorphisms were detected by ligase detection reaction （LDR）, including rs574584, rs1048101, rs3739216 and rs3802241. The frequency of genotypes and haplotype were analyzed. Results The frequencies of detected genotypes between the autoantibody against the α1-adrenergic receptor positive group and negative group were similar （P 〉 0. 05） while significant difference was in the frequencies of haplotypes （all P 〈 0.05 ）. The frequencies of genotypes with rs1048101 （genotype C/C, C/T, P = 0. 017 ） and rs3802241 （ genotype A/A, A/G, P = 0. 004 ） were significant different in autoantibody positive group compared to negative group in patients with stage 2. Conclusion ADRA1A gene polymorphism might correlate with the α1-adrenergic receptor autoantibody production in hypertensive patients.