灵龟八法“开”“闭”时辰取穴与“主”“客”顺序取穴针刺对晚孕大鼠子宫收缩活动的影响

Effects of Acupuncture at "Open" or "Close" Time and in "Host" or "Guest" Sequence on Uterine Contraction in Late Pregnancy Rats

目的:观察灵龟八法"开""闭"时辰取穴和"主客""客主"顺序取穴针刺对子宫收缩的影响,探索孕期选穴用穴的规律。方法:选怀孕Wistar大鼠32只,随机分为开主客组、开客主组、闭主客组、妊娠模型组,另设正常未孕对照组8只。按灵龟八法,开主客组、开客主组在"照海开穴"时辰,闭主客组在"非照海开穴"时辰,分别针刺"照海"穴、"列缺"穴各5 min,再针刺"合谷"穴20 min,之后针刺"三阴交"穴5 min,观察子宫张力和子宫收缩频率的变化。结果:与正常未孕对照组比较,妊娠模型对照组及3个针刺组的子宫张力和子宫收缩频率大多显著增高(P<0.01,P<0.05)。与妊娠模型对照组比较,闭主客组的子宫张力在第8时段显著降低(P<0.05);另两个针刺组的子宫张力与妊娠模型对照组比较差异无统计学意义(P>0.05)。3个针刺组的子宫收缩频率与妊娠模型对照组比较差异均无统计学意义(P>0.05)。结论:灵龟八法"闭穴"时辰、"主客"顺序取穴针刺,能减缓晚孕大鼠子宫的收缩活动,保持妊娠机体内环境的稳定。

Objective To observe the effect of acupuncture at ＂open＂ or ＂close＂ time and in ＂host＂ or ＂guest＂ sequence on uterine contraction in late pregnancy rats so as to explore the regularities of acupoint application. Methods A total of 32 pregnant rats were divided into ＂open/host-guest＂ （OHG） group, ＂open/guest-host＂ （OGH） group, ＂close/host-guest＂ （CHG） group and pregnancy group, with 8 cases in each. Eight normal rats were made up of normal control group. According to Ling Gui Ba Fa （ Eight Laws of Spirit Turtle）, acupuncture was given to ＂Zhaohai＂ （KI 6）, ＂Lieque＂ （LU 7）, ＂Hegu＂ （LI 4） and ＂Sanyinjiao＂ （SP 6） first or later separately. For example, in OHG group, KI6 （host point, being in opening time） was punctured first with the acupuncture needle manipulated for 5 min, then, LU7 （guest point） punctured and stimulated for 5 min, LI4 （corresponding point of KI6） for 20 min, and then, SP6 （another corresponding point） was punctured and stimulated for 5 min in order. In CHG group, KI6 （being in closing time） was needled for 5 min first, followed by puncturing LU7, LI4 and SP6 for 5 min, 20 min, and 5 min separately in sequence. The uterine tension and uterine contraction frequency were measured by using a tension transducer. Results In comparison with normal control group, uterine smooth muscular tension and contraction frequency increased significantly in model group, and after acupuncture stimulation in OHG, OGH and CHG groups （ P〈0.01, P〈0.05）. Compared with model group, uterine tension of OHG group was significantly lower in the 8th time course after acupuncture （ 15 - 20 min after removing acupuncture needle, P〈0.05）. No significant differences were found among model, OHG and OGH groups, and among OHG, OGH and CHG groups in uterine tension and contraction frequency （P〉0.05）. Conclusion ＂Close-host-guest needling＂ can reduce uterine tension, which is likely to have significance in maintaining a stable environment of the uterus during pregnancy.