Twist基因检测对大肠癌临床病理诊断价值的探讨

Diagnostic value of Twist gene expression in colorectal carcinoma

目的:探讨转录调节因子Twist在大肠癌组织中的表达及其在临床病理诊断中的应用价值。方法:应用鼠抗人Twist单克隆抗体采用SP免疫组织化学方法检测112例大肠癌组织及40例正常肠黏膜组织中Twist的表达,并与临床病理指标进行比较。结果:在112例大肠癌组织中,Twist基因阳性表达78例(69.6%),正常组织中为15例(17.5%),两者比较差异有统计学意义,χ2=11.21,P=0.001;进一步结合临床病理资料分析,Twist在大肠癌组织中表达与肿瘤的分化程度(χ2=9.575,P=0.008)、肿瘤浸润(χ2=8.183,P=0.017)、淋巴结转移(χ2=4.05,P=0.044)和Duke分期(χ2=11.114,P=0.011)有关;而与患者年龄(P=0.286)、性别(P=0.887)无关。结论:Twist基因表达对大肠癌临床病理学诊断有明确的指导作用。

OBJECTIVE：To investigate the expression of Twist in colorectal carcinoma and identify the clinical and pathological use of it. METHODS： The expressions of Twist were detected by immunohistochemical SP method in 112 cases of colorectal carcinoma and 40 cases of normal colorectal tissues from surgical resections. RESULTS： The positive rate of Twist in colorectal carcinomas was 69.6% while the positive rate in normal gastric tissues was 17.5%, χ2=11.21,P=0.001, Twist overexpression was related with the tumor differentiation （χ^2=9.575,P=0.008）, the depth of invasion （χ^2=8.183,P=0.017）, lymph node metastasis （χ^2=4.05,P=0.044） and Duke staging （χ^2=11.114,P=0.011）, while not related with age （P=0.286） and sex （P=0.887）. CONCLUSION： Twist may play an important role in the formation and development of colorectal carcinomas. The expression of Twist is a useful marker in the clinical and pathological diagnosis of colorectal carcinomas.