胰腺癌及胰腺星状细胞中基质细胞衍生因子SDF-1及其受体CXCR4的表达

The expression of stromal cell-derived factor-1 and CXCR4 in pancreatic carcinoma tissues, cell lines and pancreatic stellate cells

目的检测基质细胞衍生因子（SDF-1）及其受体CXCR4在胰腺癌组织、细胞株及星状细胞（PSC）中的表达。方法采用免疫组织化学方法检测37例胰腺癌及10例癌旁正常胰腺组织SDF-1、CXCR4、α-SMA蛋白表达以及细胞株AsPC-1、PSC的SDF-1、CXCR4蛋白表达。RT—PCR检测AsPC-1、BxPC3、SW1990及PSC的SDF-1、CXCR4 mRNA表达。结果37例胰腺癌CXCR4表达（＋）8例、（＋＋）20例、（＋＋＋）9例；10例癌旁正常胰腺组织CXCR4表达（-）2例、（＋）7例、（＋＋）1例，两者差异显著（P〈0．01）。胰腺癌的间质组织SDF-1的表达高于癌旁间质组织（P〈0．01），并随α—SMA表达的增加而增加。胰腺癌细胞株AsPC-1有CXCR4蛋白表达，而PSC有SDF-1蛋白表达。AsPC-1、BxPC3、SW1990细胞株均有CXCR4 mRNA的表达，而无SDF-1 mRNA的表达；PSC有SDF-1 mRNA表达，CXCR4 mRNA微弱表达。结论胰腺癌组织及细胞系表达CXCR4，PSC表达SDF-1，PSC有可能通过SDF-1／CXCR4轴促进胰腺癌的侵袭转移。

Objective To investigate the expressions of stromal cell-derived factorl （SDF-1） and its receptor CXCR4 in human pancreatic carcinoma tissues, cell lines and pancreatic stellate cells （PSCs）. Methods SDF-1 /CXCR4 and α-SMA protein expression levels and SDF-1 and CXCR4 protein in AsPC-1 and PSCs were detected by immunohistochemical staining in 10 cases of peri-carcinoma tissues and 37 cases of pancreatic carcinoma tissues. The expression of SDF-1 and CXCR4 mRNA in pancreatic cell lines and PSCs were detected by RT-PCR. Results CXCR4 were positively expressed in all pancreatic carcinoma tissues [ （ ＋ ） 8 cases, （ ＋ ＋ ） 20 cases, （ ＋ ＋ ＋ ） 9 cases] ; and there were no CXCR4 expression in 2 cases of peri-carcinoma tissues and CXCR4 were positively expressed in 8 cases [ （ ＋ ） 7 cases, （ ＋ ＋ ） 1 cases] ; with significant difference （P 〈 0.01 ）. And the expression of SDF-1 protein in carcinomatous stromal tissues was much higher than that in the stromal tissues of peri-carcinoma （P 〈 0.01 ）, and it corresponded to the increase of α-SMA expression. The CXCR4 protein expression was found in AsPC-1, while SDF-1 protein expression was found in PSC. The CXCR4 mRNA expression was found in AsPC-1, BxPC3, SW1990, while there were no SDF-1 mRNA expression in the above mentioned cell lines. SDF-1 mRNA was expressed in PSC and CXCR4 mRNA was weakly expressed in PSC. Conclusions The expression of CXCR4 mRNA and protein were found in pancreatic carcinoma specimens and cell lines. PSCs expressed SDF-1 mRNA and protein. PSCs may promote the invasion and metastasis through SDF-1/CXCR4 axis.