胰腺癌HMGB1表达及其与血行转移的关系研究

Relationship between the expression of high mobility group protein B1 and blood-borne metastasis in pancreatic carcinoma

目的探讨人胰腺癌高迁移率族蛋白B1（high mobility group protein B1，HMGB1）表达及其与血行转移的关系。方法应用Western blot法检测68例胰腺癌患者、18例CP和21例健康者血清HMGB1水平，并对其中37例胰腺癌患者手术前后的血清HMGB1水平进行比较；应用免疫组织化学法检测67例胰腺癌组织HMGB1和CD31的表达。结果胰腺癌、CP及健康者血清HMGB1水平分别为（119．7±54．5）ng／ml、（40．2±25．5）ng／ml和（13．1±4．3）ng／ml，相差非常显著（P〈0．001）。胰腺癌患者术后血清HMGB1水平为（69．3±5．1）ng／ml，显著低于术前的（120．2±8．2）ng／ml（P〈0．001）。胰腺癌组织HMGB1表达阳性率为43．6％，HMGB1表达与组织分化、TNM分期及转移有关，P均〈0．01；HMGB1表达与血管密度呈显著正相关（r=0．76，P〈0．0001），免疫组化显示，HMGB1表达阳性的肿瘤细胞多位于有腔血管周围，位于血管内的肿瘤细胞HMGB1阳性表达率为71％。结论胰腺癌患者HMGB1呈高表达，表达HMGB1的肿瘤细胞易于进入血管内，与其血行转移有关。

Objective To investigate the correlation of the expression of high mobility group protein B1 （HMGB1） and blood-borne metastasis in patients with pancreatic carcinoma. Methods The serum HMGB1 levels were determined by Western blot analysis in 68 patients with pancreatic cancer, 18 patients with chronic pancreatitis and 21 healthy controls. Among the 68 patients with pancreatic cancer, the serum HMGB1 levels of 37 patients before and after operation were compared. The expression of HMGB1 and CD31 were detected by immunohistoehemistry in 67 patients with pancreatic carcinoma. Results The serum HMGB1 levels in patients with pancreatic cancer was （ 119.7 ± 54.5 ） ng/ml, which was significantly higher than （40.2 ± 25.5 ） ng/ml in chronic pancreatitis （ P 〈 0. 001 ） and （ 13.1 ± 4.3 ） ng/ml in healthy control （ P 〈 0. 001 ）. The serum HMGB1 levels in patients with pancreatic cancer before operation was （ 120.2 ± 8.2） ng/ml, which was significantly higher than （69.3 ± 5.1 ） ng/ml after operation （ P 〈 0. 001 ）. The expression rate of HMGB1 in pancreatic cancer tissues was 43.6%. The expression of HMGB1 were signifieantly related with tumor differentiation, TNM stage, blood-borne metastasis, and vascular density（P 〈 0.01 ）. The HMGB1 positive tumor cells were adjacent to the blood vessels with lumen, and the rate of HMGB1 expression in intravascular tumor cells was 71%. Conclusions The HMGB1 was highly expressed in panereatie cancer tissues. HMGB1 expression positive pancreatic carcinoma cells were prone to invade blood vessels with lumen which may be related to blood-borne metastasis in pancreatic cancer.