亚麻中雄性不育基因同源序列MS2-F的克隆和表达分析

Cloning and Expression Analysis of Male Sterility Gene Homology Sequence MS2-F in Flax

用同源序列克隆法从亚麻中克隆了雄性不育基因同源序列MS2-F cDNA(登陆号:EU363493)。该cDNA全长1911bp,包含一个1608bp的ORF,编码535个氨基酸。推导的蛋白质序列中包含2个雄性不育保守区:NAD结合区域和雄性不育C-末端区域。该基因与油菜和拟南芥雄性不育基因的一致性分别为59.65%和59.16%,为花蕾特异表达基因,推测在亚麻花粉发育过程中与脂酰辅酶A还原酶有相似功能。MS2-F cDNA对应的gDNA大小为2696bp(登陆号:EU365361),含有8个内含子和9个外显子。

A cDNA, homologous to MS2 in Arabidopsis thaliana, named MS2-F （GenBank accession No.： EU363493）, was cloned from flower buds of flax by Homology-based cloning method. The complete cDNA sequence was 1 911 bp and contained an ORF of 1 608 bp, which encoded a protein of 535 amino acids including two male sterile conserved domains： NAD-binding domain and male sterile C-terminal domain. MS2- F amino acid sequence identities to MS2Bnap in Brassica napus and MS2 in Arabidopsis thaliana were 59.65% and 59.16% respectively. The gene was exclusively expressed in flower buds and may act as an acyl CoA reductase during flax pollen development. Genomic DNA fragment of the gene was 2 696 bp （GenBank accession No.： EU365361） and contained 8 introns and 9 exons.