乙肝病毒C基因亚型与病毒变异的相关研究

Study on the Relationship between HBV C Sub-Genotype and Viral Mutations

目的探讨乙肝病毒C基因亚型与病毒变异的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法,对155例慢性HBVC基因感染者,包括78例肝细胞癌(HCC)、36例肝硬化(LC)和41例慢性肝炎(CH)血清样本进行C基因亚型和病毒基本核心启动子(BCP)以及前C区(PC)变异检测。结果在HCC、LC和CH研究组中C1的分布均高于C2,同时C1亚型有较高的BCP变异,C2亚型有较高的PC变异(P<0.030)。结论慢性HBVC基因型患者HCC的高发生率和有较严重的肝脏损害可能与其中C1分布较多有关,因此HBVC基因型患者进行亚型鉴定能更好地评估HBV基因型系列对慢性HBV感染疾病进展和临床结局的影响。

Objective To investigate the relationship between HBV C sub-genotype and viral mutations. Method A cohort of 155 patients with chronic HBV C genotype infection, including 78 cases of HCC, 36 of liver cirrhosis and 41 of hepatitis, was analyzed by polymerase chain reaction-restriction fragment length polymorphism （PCR- RFLP） or nucleotide sequencing. Result Distribution of sub-genotype C1 was higher than C2 in 155 chronic HBV infection patients. The incidence of BCP mutations was significantly higher in cases with sub-genotype C1 than that in C2 infection. Conclusion Determination of HBV C sub-genotype may be better to evaluate the progression of chronic HBV infection.