实时荧光定量PCR检测结直肠癌hPTTG1的表达及其意义

Expression of Human Pituitary Tumor Transforming Gene 1 in Colorectal Cancer Detected with Real-time Fluorescent Quantitative PCR and Its Clinical Significance

背景与目的:虽然人垂体瘤转化基因1(human pituitary-tumor transforming gene1,hPTTG1)在结直肠癌等恶性肿瘤中高表达,然而hPTTG1 mRNA与结直肠癌临床病理参数之间的关系及其能否作为结直肠癌诊断和预后判断的分子标志尚不清楚。本研究检测hPTTG1 mRNA在结直肠癌中的表达,分析其与临床病理参数之间的关系,探讨hPTTG1 mRNA作为结直肠癌诊断和转移复发的分子标志的可能性。方法:采用实时荧光定量PCR检测结直肠癌组织与对应癌旁组织hPTTG1 mRNA,并分析其与临床病理指标的关系。结果:结直肠癌组织hPTTG1mRNA表达较正常癌旁组织显著增高(0.42±0.07vs.0.03±0.01,P<0.001)。hPTTG1 mRNA与肿瘤大小、血清CEA水平有关,肿瘤直径≥3.5cm较<3.5cm显著增高(15.80±8.80vs.10.91±5.22,P<0.05),血清CEA>5ng/mL较<5ng/mL显著增高(22.79±7.42vs.9.39±2.61,P<0.001)。hPTTG1mRNA在Dukes'C、D期显著高于Dukes'A、B期(15.88±8.09、25.69±7.67vs.9.03±0.35、9.58±2.93,P<0.001)。伴有淋巴转移、肝转移或其他器官转移者(17.63±8.47、31.07±4.10和22.78±6.39)较无转移者(11.15±6.65)显著增高(P<0.001)。hPTTG1 mRNA与患者的性别、年龄和病理分型无关(P>0.05)。结论:hPTTG1 mRNA在结直肠癌中高表达,与结直肠癌的疾病进展密切相关,有助于对患者病情进展的预测。

BACKGROUND ＆ OBJECTIVE： Although human pituitary tumor transforming gene 1 （hPTTG1） is overexpressed in malignant tumors such as colorectal cancer, its correlation to clinicopathologic parameters and its value in diagnosis and prognosis prediction of colorectal cancer are still not clear. We investigated the expression of hPTTG1 in colorectal cancer tissues, and elucidated its correlation to some clinicopathologic parameters of colorectal cancer. METHODS：The expression of hPTTG1 in 60 specimens of colorectal cancer and corresponding noncancerous tissues were examined with real-time fluorescent quantitative polymerase chain reaction, and its correlation to seven clinicopathologic parameters were analyzed. RESULTS： The mRNA level of hPTI-G1 was significantly higher in colorectal cancer tissues than in corresponding noncancerous tissues （0.42±0.07 vs. 0.03±0.01, P〈0.001）, significantly higher in colorectal cancer tissues with serum CEA level of 〉 5 ng/mL than in those with CEA of 〈 5 ng/mL （22.79±7.42 vs. 9.34±2.61, P〈0.001）, significantly higher in colorectal cancer tissues with diameter of ≥ 3.5 cm than in those with diameter of 〈 3.5 cm （15.80±8.80 vs. 10.91±5.22, P〈0.05）, significantly lower in Dukes＇A, B tumors than in Dukes＇ C, D tumors （9.03±0.35 and 9.58±2.93 vs. 15.88±8.09 and 25.69± 7.67, P〈0.001）, and significantly higher in colorectal cancer tissues with lymph node metastasis （17.63 ±8.47）, liver metastasis （31.07±4.10） and other organ metastasis （22.78 ±6.39） than in those without metastasis （11.15±6.65） （P〈0.001）. hPTTG1 expression had no relationship with patients＇ age, sex and histological type （P〉0.05）. CONCLUSIONS： hPTTG1 is overexpressed in colorectal cancer. It is closely related to the progression of colorectal cancer, and may be helpful for prognosis prediction of colorectal cancer.