异小杆线虫活体繁殖技术筛选

Laboratory Screening of Technology for In Vivo Mass Production of Heterorhabditis Bacteriophora

为了筛选低成本、高质量的简单易操作的昆虫病原线虫繁殖技术,以满足不同规模市场需求。试验采用末龄黄粉虫(Tenebrio molitor)幼虫为活体寄主进行大量繁殖线虫Heterorhabditis bacteriophora(Hb),对培养介质、接种剂量、培养介质与黄粉虫混合比例、繁殖袋翻动、开始收获时间和收获后处理方法进行筛选。结果显示蛭石为低成本的培养介质,Hb最适接种剂量为黄粉虫800条/g侵染期线虫(IJs),培养介质和黄粉虫混合最适质量比例为3∶2,繁殖袋不必翻动。Hb在接种培养12d和15d进行收获,收获后经过滤处理进行储存,线虫的存活率明显高于沉淀后直接储存的线虫。

In order to meet different scale market, mass production technology for entomopatho-genic nematode of low cost and good quality was screened. Tenebrio molitor（L. ）was used as host in vivo mass production of Heterorhabditis bacteriophora （Hb）in laboratory, and culture parameters were screened.The results showed that vermiculite was better substrate, and concentration of Hb was 800 infective juveniles pre gram larvae. The proportion of substrate and the host was 3：2. Turnover bags was unnecessary. The Hb was collected at cultured 12d and 15d. The storage period of filtered nematode was longer than that of direct storage after harvest.