再生障碍性贫血与人类白细胞抗原相关性研究

Immunogenetic association of HLA class Ⅰ、Ⅱ with aplastic anemia in Chinese population

应用国际标准的微量淋巴细胞毒方法，研究了我国东北地区汉族人群人类白细胞抗原（ＨＬＡ）Ⅰ、Ⅱ类基因遗传多态性与再生障碍性贫血（ＡＡ）易感性的关系。对３１例ＡＡ患者和１０１例健康无血缘关系者对照血样本进行分析。结果表明：ＡＡ患者具有显著高的ＨＬＡ－Ｂ１３、ＤＱ２（ＲＲ＝２．２，９．３；Ｐ＜０．０１），可能是易感等位基因或其连锁的一部分；而ＤＱ３在病例与对照中呈相反结果（ＲＲ＝０．５３，Ｐ＜０．０１），可能为一拮抗等位基因，提示ＨＬＡ基因系统差异对ＡＡ发病起着重要的免疫调控作用。

Simulation studies based on immunogenetic showed. that high polymorphism at HLA loci depends on dis- ruptive selection and genes conversion in general and .he patient population. HIA class I (A,B,C) and class Ⅱ (DR , DQ ) in Chinese patients with aplastic anemia(AA) were investigated and evaluated for their role in the pathogenesis ,us- ing the microlymphocyte - cytotoxicity (MLC) . 101 Chinese unrelated controls and 31 patients with AA were examined. The results showed that the frequencies of HLA - B13, DQ2 antigenes were significantly increased (RR =2.2,9.3 ;p < 0.01 ) the frequency of HLA-DQ3 antigene was significantly decreased (RR=0.53, P<0.O1 ). The data suggest that HLA-B13, DQ2 may be markers for AA susceptible alleles while HLA-DQ3 for AA resistant allele in Chinese popula- tion.