微生物合成ε-聚赖氨酸的结构鉴定及其抑菌活性的研究

Structure Identification of ε-polylysine by Streptomyces Z-9 and Its Anti-bacteria Activity

在对土壤中筛选获得的一株白色链霉菌Z-9发酵条件初步研究的基础上,利用高效液相色谱(HPLC)、液相-质谱联用(LC-MS)、核磁共振(NMR)、凝胶渗透色谱(GPC)等分析检测方法对该白色链霉菌发酵产生的ε-聚赖氨酸进行了结构鉴定和抑菌活性研究。研究结果表明:白色链霉菌Z-9发酵产生的聚赖氨酸是由赖氨酸单体通过ε-NH2和α-COOH形成ε-酰氨键聚合而成,平均分子质量为4 210,并且对敏感菌——枯草芽孢杆菌生长有强烈的抑制作用。

Streptomyces Z-9, a ε-polylysine producing strain was isolated from soil and the fundamental fermentation behaviors of this strain was previously investigated. In this study, ε-polylysine fermentation was carried out in a 5 L bioreactor. HPLC, LC-MS, NMR and Gel Permeation Chromatography （GPC） were used for studying the structure and anti-bacteria activity of the ε-polylysine obtained. The experimental results showed that the ε-polylysine, with the average molecular weight of about 4 210 u, is polymerized by mono-lysines with the aid of amide linkages between ε-amino and α-carboxyl groups. The anti-bacteria activity experiment showed that the ε-polylysine has very strong inhibitory effect on Bacillus subtilis.