运用免疫亲和柱和高效液相色谱(HPLC)检测啤酒大麦中的脱氧雪腐镰刀菌烯醇

Detection of Deoxynivalenol in Malting Barley by Immunoaffinity Column Clear up and High Performance Liquid Chromatography(HPLC)

采用免疫亲和柱净化、高效液相色谱定量等方法对啤酒大麦中的脱氧雪腐镰刀菌烯醇(DON)进行检测。向啤酒大麦中加入标准品,加标的范围在0.10～2.00μg/g,加标回收率为92.04%～97.29%,相对标准偏差是0.92%～2.67%。检出限是0.01μg/g。所测样品中DON的含量范围在0.0123～0.0926μg/g,其中江浙、云南和东北产区的啤酒大麦中DON含量较高。

An analytical study was conducted based on immunoaffinity column clear up, and High Per-formance Liquid Chromatography for purification and quantity detection of Deoxynivalenol in malting barley, With malting barley addition level from 0.10μg/g to 2.00μg/g, recoveries of Deoxynivalenol from malting barley spiked ranged from 92. 04%- 97. 29%, and relative standard deviation was between 0. 92% and 2.67%. The limit of detection was 0. 01μg/g. Of the total samples that contained DON, concentration ranged from 0. 0123 to 0. 0926μg/g. A higher level of DON was found in the malting barely from Jiangzhe, Yunnan and Northeast.