控制水稻品种Koshihikari抽穗期的数量性状位点

Quantitative Trait Loci for Rice Heading Time in Koshihikari

利用Koshihikari×Kasalath BILs群体及相应的Kasalath/Koshihikari CSSLs群体,在江苏南京和海南陵水两个环境下对抽穗期的QTL定位分析。结果表明,在两地重复检测到3个QTL,分别位于第3、6和8染色体上;在qHd-3和qHd-8位点,来自Koshihikari等位基因能够提早抽穗,在qHd-6位点,来自Koshihikari等位基因能够延迟抽穗;第3染色体qHd-3和第7染色体非加性QTL之间存在上位性互作,通过重组自交系和置换系相互验证发现,qHd-3所在的标记区间与W008的Kasalath插入片段位置大体一致,qHd-8所在的标记区间与W023、W024的Kasalath插入片段相吻合,qHd-7-1所在的标记区间位于W20的Kasalath片段之内,表明确实存在这3个位点。同时还对Koshihikari×桂朝2号RILs抽穗期的QTL定位分析,在江苏南京和海南陵水分别检测到3个加性抽穗期QTL,1对非加性抽穗期QTL存在互作。

In order to verify the quantitative trait loci for rice heading time in Koshihikari, QTL mapping of heading time was studied using both Koshihikari/Kasalath BILs population and Kasalath/Koshihikari CSSLs population in Nanjing, Jiangsu province and Lingshui, Hainan province. The results indicated that three QTL of heading time were detected, which were located on chromosomes 3, 6, and 8, respectively. At qHd-3 and qHd-8 loci, the allde from Koshihikari can promote heading time for rice, but at qHd-6-1 loci, the allele from Koshihikari could delay heading time for rice. Epistatic interaction was detected between qHd-3 and the QTL located on chromosome 7. Comparing BILS with CSSLs linkage maps, we found that the marker regions of qHd-3, qHd-8, and qHd-7-1 coincided with Kasalath segment in W008, W023 and W024, W020, respectively. Meanwhile, QTL mapping of heading time were studied using Koshihikari/Guichao 2 RILs population, three additive QTL were detected and epistatic interaction was detected between two unadditive QTL of heading time.