小麦高分子量谷蛋白亚基功能的体外鉴定

In vitro Functional Testing of High Molecular Glutenin Subunits in Wheat

通过SDS-PAGE方法回收纯化小麦高分子量谷蛋白亚基1Ax1、1Dx5、1Dy10、1Bx7、1By8和1By9,然后利用微量配粉方法将单个亚基分别添加到对照"京411"面粉中,经过揉混仪分析单个亚基对揉面特性的影响,进而确定各个亚基的功能特性。根据揉面时间、稳定时间等参数的变化,6个小麦高分子量谷蛋白亚基对面粉品质的影响表现为1Dy10>1Ax1>1Dx5>1By8>1Bx7>1By9。研究结果表明,通过揉混仪进行体外配粉检测是快速鉴定高分子量谷蛋白亚基功能的一种有效方法。

The functions of high molecular weight glntenin snbunit （HMW-GS） can be analyzed by determining the parameters subject to wheat （Triticum aestivum L.） quality. However, to date, only several glutenin subunits have verified in their functions despite a lot of subunits designated. Therefore, a simple and rapid method is highly needed to detect the functions of wheat glu- tenin subunits. The 2 g and 10 g Mixograph have been applied in related studies in Australia and other countries. To determine the function of wheat single HMW-GS, 6 subunits, 1Axl, 1DxS, 1Dyl0, 1Bx7, 1ByS, and 1By9, were separated and purified by SDS-PAGE, and then mixed into flour of Jing 411 individually. Their effects on flour mixing properties were investigated by 10 g Mixograph testing. The tested mixing parameters were mixing time, stability time, peak resistance, peak width, right slop, and width at 8 rain. Four kinds of solution were applied to obtain an appropriate control, and the solutions consisted of different com- binations of 0.2% acetic acid, 50μg mL-1 DTT, 200 pg mL-1 KIt3, and 0.1% SDS. When signal subunit was added into dough, 6 mixing parameters changed to a different degree. The effects of the 6 subunits on flour quality were ranked as 1Dy10 〉 1Axl 〉 1Dx5 〉 1By8 〉 1Bx7 〉 1By9 according to mixing time, and 1Axl and 1Dyl0 〉 1Dx5 〉 1By9 〉 1Bx7 〉 1By8 according to sta-bility time, The peak resistance of 1By8 was the biggest among that of all subunits. The right slop varied little among that of the 6 subunits. The width at 8 min of 1Bx7 was the smallest among that of all subunits, The 1By8 was primarily considered as a good quality subunit in this study. In addition, it is suggest that in vitro small-scale testing used in this study is an effective method to study the function of single HMW subunit.