几种因素对银杏毛状根诱导和黄酮含量的影响

Effects of Several Factors on Hairy Root Inducement and Flavone Content of Ginkgo biloba

考察了发根农杆菌菌种、侵染时间、外植体类型、培养基类型和乙酰丁香酮5个因素对银杏毛状根诱导的影响,以及比较了后二因素对毛状根黄酮积累的影响。结果表明:以银杏胚为外植体,A4活化菌液中加入乙酰丁香酮100μmol.L-1侵染15 min,再在MS培养基上培养发根率最高。乙酰丁香酮对毛状根中黄酮的积累没有显著促进作用,浓度高于120μmol.L-1还会严重抑制发根。

Five factors which influence inducement of Ginkgo biloba hairy roots, including Agrobacterium rhizogenes types,intrusion time,explant types, medium types and AS were reviewed in this article. The effect of the two later factors on flavones content in hairy roots was also compared. The results showed that：Rooting rates was the highest in the track that embryo intruded by activity A4 with 100 mol · L^-1 AS for 15 minutes and then cultured on MS medium. There was no markedly simulative function of AS for flavone accumulation in hairy roots. At the same time,rooting rate was badly restrained when AS content was higher than 120 mol·L^-1.