摘要
通过多次实验研究,力图找出不同基本培养基和不同浓度激素对铁皮石斛组织快繁时愈伤组织诱导的影响。研究结果表明:诱导愈伤组织时,在MS、B5、N6、RM这4种基本培养基中,以N6基本培养基对愈伤组织的诱导效果最好;添加4种不同浓度的6-BA,浓度为1.0 mg.L-1时诱导愈伤组织效果最好。
The study was striving to find out the effect of inducing of callus formation during the rapid propagation in Dendrobium candidum, with different basical culture medium and concentration of hormones. The results showed that the optimal inducing medium was made of N6 among the different inducing medium of MS, B5, N6, RM when inducing of callus formation, moreover, the best effort was adding 1 mg · L^-1 of 6-BA among the four varies concentration.
出处
《黑龙江农业科学》
2008年第6期19-20,共2页
Heilongjiang Agricultural Sciences
基金
南阳师范学院项目(nyct200405)
关键词
铁皮石斛
组织快繁
愈伤组织
Dendrobium candidum
tissue culture
callus
