人肝细胞毛细胆管表达IL-2Rα的研究及意义

Expression and characterization of IL-2R_α on cholangiole of human hepatocyte

目的了解人肝细胞毛细胆管是否表达IL-2Rα,观察其表达特异性和灵敏度。方法对正常人肝组织、慢性乙型肝炎等不同病种人肝组织进行IL-2Rα标记,通过免疫组织化学染色、免疫荧光化学染色及免疫电镜3种方法观察其表达。结果免疫组化染色法和免疫荧光染色法显示IL-2Rα标记人肝细胞膜毛细胆管面定位正确,无非特异性染色,而肝细胞膜的血窦面、细胞质、细胞核不被IL-2Rα标记。酶标记免疫电镜显示人肝细胞膜毛细胆管面有明显电子致密物沉积。结论人肝组织毛细胆管确实表达IL-2Rα,其特异性和灵敏度均优于CEA(多克隆抗体)。

Purpose In order to observe if cholangiole of human hepatocyte can be signed by IL-2Rα or not and investigate its expression specificity and sensitivity.Methods Hepatic tissues from normal human liver and patients with different diseases such as chronic hepatitis B,primary biliary cirrhosis（PBC） and so on,to observed the expressions of IL-2Rα on cholangiole of human hepatocyte by the ways of immunohistochemisty,immunofluorescence and Immunomicroscope.Results Cholangiole of human hepatocyte can be accurately signed by IL-2Rα through the ways of immunohistochemisty and immunofluorescence but the other membrane,cytoplasm and nucleus of human hepatocyte can not be signed by IL-2Rα.There were clear high electron density on the cholangiole of human hepatocyte by the way of immunomicroscope.Conclusions Cholangiole of human hepatocyte does express marker IL-2Rα.IL-2Rα can be used as new marker for cholangiole of human hepatocyte.IL-2Rα staining showed better specificity and sensitivity than CEA（polyclone antibody） marker.