摘要
NAC转录因子是近些年来新发现的植物特有的转录调控因子,其N端含有高度保守的NAC结构域,在植物的生长发育、器官建成、逆境胁迫以及作物的品质改良中具有重要作用。通过构建优质出口型大花生‘鲁花14’开花后20～60d不同发育时期的种子混合cDNA文库,克隆到一个花生NAC类基因AhNAC1（GenBank登录号为EU669863）。序列分析表明,AhNAC1基因的cDNA序列长度为1453bp,其开放阅读框长度为912bp,编码303个氨基酸,预测其分子量为34.1kD,等电点为7.6。通过与其它植物的NAC转录因子的蛋白序列比对,发现AhNAC1蛋白属于ATAF亚族,可能在植物种子发育及逆境反应中起作用。此花生NAC类基因属首次报道。
NAC transcription factors are the new type transcription regulatory factors discovered in recent years which are unique to plants. They contain a highly conserved NAC domain in N-terminal ends. It is reported that NAC transcription factors play an important role in multiple biological functions such as development, organs foundation, responses to stresses, and improving of crops quality. By constructing the cDNA library of different stages （20 to 60 days after flowering） seeds of peanut cultivar ＇Luhua 14＇ ,we cloned a NAC-like gene AhNAC1 （ has a length of 1 453 bp w mino acids with an estimat NAM members, AhNAC1 ment,as well as in stress r GenBank accession number EU669863）. Sequence analysis shows that the cDNA hich contains a single open reading frame of 912 bp encoding a protein of 303 aed molecular mass of 34.1 kD and an isoelectric point of 7.6. Compared to other belongs to ATAF subfamily and may play multiple roles in the seed developesponse pathways. This is the first NAC-like gene reported in peanut.
出处
《西北植物学报》
CAS
CSCD
北大核心
2008年第10期1929-1934,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
科技部“863”计划重点项目(2006AA10A114)
关键词
花生
NAC
克隆
功能预测
peanut
NAC
clone
function prediction
