苹果属山荆子遗传多样性的RAPD分析

RAPD Analysis of Genetic Diversity of Malus baccata(L.) Borkh

采用RAPD分子标记对东北、华北地区山荆子8个天然居群的137株个体进行了遗传多样性研究,10个引物共得到72个扩增位点,其中多态性位点63个。多态位点百分率为87.50%,有效等位基因数(Ne)为1.6021,Nei’s基因多样性(H)为0.3386,Shannon信息指数(I)为0.4961,表明山荆子遗传多样性水平较高。基因流(Nm)为1.7353,说明山荆子各居群间存在一定的基因交流。居群间基因分化系数(Gst)值为0.2237,说明虽然山荆子居群的遗传变异主要存在于居群内,但各居群间也存在着较高的遗传分化。

The randomly amplified polymorphic DNA（RAPD） technique was adopted to examine genetic diversity and structure of 8 populations of Malus baccata in North China area with 137 individual plants and 72 amplification sites from 10 primers,which included 63 polymorphic sites. The results showed that percentage of polymorphic loci（PPB） was 87.5% ,effective number of alleles （Ne） was 1. 602 1 ,Nei＇s gene diversity （H） was 0. 338 6, Shannon＇s information index （I） was 0. 496 1, which indicated that M. baccata had high genetic diversity. Gene flow among populations （Nm） was 1. 735 3,which proved that there was some intercommunion among M. baccata populations. The coefficient of gene differentiation （Gst） was 0. 223 7. The results showed that although mostly genetic variability was attributed to the differentiation within populations,there were high gene differentiations among populations simultaneity.