赤霉素和烯效唑对东北红豆杉紫杉醇代谢的影响

Effect of GA_3 and Its Synthesis Inhibitor Sumiseven on the Taxol Metabolism of Taxus cuspidata

以一年生东北红豆杉扦插苗为材料,采用Hoagland营养液添加赤霉素及其合成抑制剂烯效唑的方法,研究了不同处理对东北红豆杉紫杉醇（taxol）及其前体巴卡亭Ⅲ（baccatinⅢ）与10-去乙酰基巴卡亭Ⅲ（10-DAB）含量变化的影响,同时比较了苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）和超氧化物酶（SOD）活性变化,分析了紫杉醇合成旁路途径物质及抑制剂对紫杉醇代谢的作用效应。结果表明,不同浓度赤霉素（GA3）和烯效唑（S3307）处理对东北红豆杉POD、SOD及PAL活性的影响不显著;但整个处理过程中叶片中紫杉醇含量均为对照的1.28～6.44倍,茎中紫杉醇、baccatinⅢ及叶片中10-DAB含量在第6天均高于相应对照;赤霉素与烯效唑对紫杉醇及其前体合成的作用途径存在一致性。

With one-year-old seedling as treatment materials, we investigated the content of taxol, baccatin Ⅲ and 10-DAB and the activities of PAL,POD and SOD by addition of different centigrade of sumiseven and gibberrellin in culture solution,in order to assess the metabolism of taxol. The result proved that when treated with different concentrations of GA3 and S3307, there were no obvious differences between treatments and control. However,the content of taxol in leaves was higher than that of control during the peri- od,which were 1.28 to 6.44 times higher,and the contents of taxol and baccatin Ⅲ in stem, 10-DAB in leaves on the 6th day also higher than that of control. The results indicated that the effects of GA3 and Sa307 on taxol and its＇ precursors synthesis are consistent.