摘要
目的探讨重组人干扰素α-2b(rhIFNα-2b)对体外培养的腭裂术后裸露骨面瘢痕来源的成纤维细胞生物学作用的影响。方法制作兔腭裂术后裸露骨面瘢痕动物模型,利用体外细胞培养技术,通过MTT法、3H-TDR渗入法、流式细胞术等方法观察、分析rhIFNα-2b对腭裂术后瘢痕成纤维细胞生物学行为的影响,数据采用方差分析及q检验处理。结果与对照组比较,实验组加入rhIFNα-2b后,腭裂术后瘢痕成纤维细胞的生长趋势及增殖活性及被明显抑制,G1期细胞百分数明显增高。结论rhIFNα-2b对腭裂术后瘢痕成纤维细胞的生长趋势及增殖活性具有明显抑制作用,说明在防治腭裂术后裸露骨面瘢痕的形成中有一定的应用价值。
Objective To study the biological activities of rhIFNα-2b on the fibroblasts from palatal scar.Methods Making rabbit palatal scar animal model,palatal scar derived fibroblasts cultivated in vitro were observed and analysed through MTT tests,3H-TDR tests and flow cytometry（FCM）.Then the effects of rhIFNα-2b on the biological behavior of palatal scar fibroblasts were determined.The data was analysed by statistical analysis of variance and q test.Results Compared with the control group,the experimental rhIFNα-2b group had changes on inhibition of fibroblasts growth and proliferation,increase of the G1 phase cell percentage.Conclusion rhIFNα-2b can inhibit growth and proliferation of palatal scar fibroblasts.It has beneficial effect in the treatment of palatal scar.
出处
《安徽医科大学学报》
CAS
北大核心
2008年第5期520-523,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省自然科学基金项目(编号:70413105)
安徽省教育厅自然科学基金项目(编号:2001kj136)
安徽医科大学博士科研基金(编号:XJ200704)
安医大校基金(编号:2006kj24)
