桂花品种资源的遗传多样性分析

Study on the Genetic Diversity of Osmanthus fragrans Cultivars

根据桂花(Osmanthus fragrans)品种的形态特征,结合AFLP分子标记,对部分桂花栽培品种进行了遗传多样性分析。结果表明,桂花品种之间存在着较为丰富的遗传多样性,AFLP分子标记检测到的多态性条带占总扩增条带的57.46%。根据桂花品种的主要性状特征,利用数值分类法对其进行分类,并用UPGMA法对AFLP结果进行聚类分析,结果均显示桂林地区的桂花品种存在明显的区域性,花色可以作为重要的分类标准,同时对桂花品种的分类系统进行了探讨。

We used morphological features and amplified fragment-length polymorphism （AFLP） markers to analyze the genetic diversity of 45 Osmanthus fragrans cultivars and found high levels of genetic diversity among the cultivars. Of the AFLP bands detected, 57.46% are polymorphic. We classified the cultivars biometrically according to their major morphological features and performed cluster analysis of the AFLP data by the unweighted pair-group method using arithmetic averages. The results of both approaches showed that O. fragrans cultivars of the Guilin region form a distinct subgroup, and flower color is a viable classification criterion. We discuss systems for O. fragrans cultivar classification.