摘要
以泥鳅(Misgurnus anguillicaudatus)为研究对象,研究不同重金属离子(Cd、Pb、Zn)在不同暴露时间(1、2、4、7、14、21、28、35 d)、不同暴露浓度(0.05、0.5、5.0 mg/L)下对泥鳅肝胰脏ATPase和SOD活性的影响.结果显示,处理组中ATPase和SOD活性分别为0.300~1.820和0.206~2.477;活性变化表现为先下降后上升再下降;低浓度处理组(Cd0.05和Zn0.5对ATPase;Cd0.05组对SOD活性在1 d时)对它们的活性有极显著诱导作用(P〈0.01),高浓度处理组(Cd5.0、Pb0.5和Pb5.0组对ATPase;Pb5.0、Zn0.5和Zn5.0对SOD活性在1 d时)则表现为显著抑制作用(P〈0.05);混合重金属离子间相互作用十分复杂,既有协同作用也有拮抗作用,但均表现为毒性增强作用,对ATPase和SOD活性的诱导作用减弱,抑制作用增强.
Using Misgurnus anguillicaudatus as an experimental animal,the effects of heavy metals Cd, Pb and Zn on ATPase and SOD activities in hepatopancreas were studied when treated for different days(1, 2, 4, 7, 14, 21, 28, 35d) and by different concentrations(0.05, 0.50, 5.00 mg/L). The results showed that ATPase and SOD activities were from 0. 300 to 1. 820 and from 0. 206 to 2. 477 respectively, and the change tendency was generally descended first, then ascended and finally descended again; ATPase and SOD activities were mostly induced in the low concentration treated groups(Cd0.05 and Zn0.5 to ATPase; Cd0.05 to SOD at the first day)(P〈0.01), but decreased in the high concentration treated groups(Cd5.0, Pb0.5 and Pb5.0 to ATPase; Pb5.0, Zno.s and Zn5.0 to SOD at the first day)(P〈0. 05); joint-experiment effects of ions were much more complex and mainly manifested toxicity in build up. They played synergetic or antagonistic actions according to the treated time, and each of which didmore harm to the enzyme activities on M. anguillicaudatus. They could weaken the inducement of ATPase and SOD activities and enhance the decrease of the ATPase and SOD activities.
出处
《甘肃科学学报》
2008年第3期55-59,共5页
Journal of Gansu Sciences
基金
国家自然科学基金项目(30470320)