血清灭活法对消除ELISA假阳性结果的比较研究

The Comparative Study on Eliminating The False Positive Result of ELISA by The Method of Inactivated Serum

目的建立一种简便、易行的方法,对酶联免疫吸附试验的结果进行质量控制。方法依据国标ELISA方法对578份小鼠血清进行11种病毒抗体检测,共检测抗体3677份。对初步检测出的242份抗体阳性血清,经56℃30min水浴灭活处理后进行复检。结果可疑阳性血清经灭活处理后复检,正常抗原孔A值和特异抗原孔A值明显低于初检的正常、特异抗原孔A值,差异显著(P<0.05);初检和复检的特异抗原孔A值与正常抗原孔A值的比值有显著性差异(P<0.05);血清灭活前后阳性率有显著性差异(P<0.01),分别为6.58%和2.86%。阳性对照灭活前后A值无显著差异(P>0.05)。结论血清经灭活处理后,使检测结果的假阳性率显著降低。

Objective Establish one kind of simple and easy procedure to carry out the quality control to ELISA result. Methods Detect 3677 antibodies of 11 murine viruses to 578 serum of mouse on the basis of GB ELISA, carry out duplicate detection on the 242 positive serum in the initial detection after 56℃ 30 min water bath inactivation processing. Result Duplicate detection on the probable positive serum after inactivation processing. The normal antigen pore A numerus and special antigen pore A numerus obtained in the duplicate detection are obviously lower than that obtained in the initial detection respectively, with a remarkable difference （ P 〈 0.05 ） ; The special antigen pore A numerus in initial and duplicate detections are very different （ P 〈 0.05） from the normal antigen pore A numerus. The positive rates before and after serum deactivation are very different （P 〈 0.01 ）, 6.58 % and 2.86 % respectively. There is no remarkable difference between the A humerus of positive control before and after inactivation （ P 〉 0.05 ）. Conclusion The false positive rate is detected to be much lower after the inactivated processing of serum.