多种辅助方法对兔肝脏射频热消融效果的影响

Effect of different methods on RFA treatment of rabbit liver

目的通过实验兔肝脏射频消融(RFA)实验,评价生理盐水、四氧化三铁纳米以及肝动脉夹闭对RFA效果的影响作用。方法清洁级新西兰兔44只,每组11只,共4组进行实验。A组(对照组):单纯RFA;B组:射频区内注射生理盐水+RFA;C组:射频区内注射2%四氧化三铁纳米液2 ml+RFA;D组:手术夹闭肝动脉+RFA。按分组方法进行准备后,进行射频消融。术后一周处死动物,测量消融区面积并进行病理检查,对结果进行统计学分析。结果消融灶A组(1.06±0.37)cm^2,B组(1.94±0.76)cm^2,C组(1.41±0.51)cm^2,D组(2.45±0.69)cm^2,B、D各组与对照组比较差异有极显著统计学意义(P=0.001和P=0.0001)。病理检查显示射频区完全坏死率A组27.3%,B组63.6%,C组36.4%,D组72.7%,B、D各组与对照组比较差异有显著统计学意义(P=0.036)。结论肝脏射频消融联合生理盐水注射以及夹闭肝动脉后射频消融较单纯射频消融能获得更大的毁损区。

Objective To evaluate the effect of three different methods on the radiofrequency ablation （RFA） treatment of rabbits liver. Methods Fourty-four rahbits were divided into 4 groups., group A, single RFA, group B, RFA combined with 2 ml normal saline injetion, group C, RFA combined with 2% nano-sized Fe3O4 liquid 2 ml, and group D, RFA combined with liver artery clipping. The rabbits were killed one week after the therapy, the size of necrosis was accounted and pathologically observed. Results The size of the RFA was （1.06±0.37）cm^2 in group A, （1.94±0.76）cm^2 in B, （1.41±0.51）cm^2 in C and （2.45±0.69）cm^2 in group D. Significant difference existed either between group B to A or group D to A （P=0. 001 and P=0. 0001, respectively）. The complete necrosis rate was 27.3%, 63.6%, 36.4%, and 72.7% of group A, B, C, D, respectively, and significant difference was also observed either between group B to A or group D to A （P= 0. 036）. Conclusion RFA combined with NS injection and with liver artery clipping is helpful to increase the therapeutic effect of RFA of rabbits liver. [