凝集素样氧化低密度脂蛋白受体-1介导氧化低密度脂蛋白促进血管平滑肌细胞血管紧张素转化酶的表达

ox-LDL enhances ACE mRNA expression of vascular smooth muscles cells via LOX-1 receptor

目的：观察氧化低密度脂蛋白（ox-LDL）对血管平滑肌细胞（VSMC）血管紧张素转化酶（ACE）表达的影响及凝集素样氧化低密度脂蛋白受体-1（LOX-1）在其中的作用。方法：人脐带动脉平滑肌细胞体外原代培养,传至4～5代用于实验。ox-LDL干预,RT-PCR测定VSMC ACE mRNA和LOX-1mRNA表达。并观察予以LOX-1的抑制剂多聚肌苷酸作用后,VSMC ACE mRNA表达的变化。结果：①较高浓度ox-LDL（20mg/L,100mg/L）作用后,VSMC ACE mRNA表达升高,作用1h出现峰值。低浓度ox-LDL（10mg/L）此种作用不明显。②ox-LDL作用后,VSMCLOX-1 mRNA表达升高,且随着ox-LDL浓度的升高而表达增强。③多聚肌苷酸抑制LOX-1后,ACE mRNA表达明显下降。结论：ox-LDL明显促进VSMC ACE表达,LOX-1在此过程中起重要介导作用。

Objective： To observe the effect of oxidized low density lipoprotein （ox-LDL） and lectin-like low density lipoprotein receptor-1 （LOX-1） on angiotensin converting enzyme （ACE） mRNA expression of vascular smooth muscles cells （VSMC）. Method：Smooth muscle cell from human umbilical artery was cultured in vitro and 4-5th generation was taken for use. Interfered in ox-LDL, the quantity of VSMC ACE mRNA and LOX-1 mRNA were detected by RT PCR. And the changes of VSMC ACE mRNA expression was observed after treatment of polyinosinic acid which was an inhibitor of LOX1. Result： （1）The quantity of VSMC ACE mRNA increased after co-cultured with higher concentration ox LDL（20 mg/L, 100 mg/L）, and the peak value was found at about the lth hours. No obvious influence was found for low concentration ox-LDL（10 mg/L）. （2）The quantity of VSMC LOX-1 mRNA increased significantly with dependence on ox-LDL concentration. （3）ACE mRNA was reduced significantly when LOX-1 was inhibited by polyinosinie acid. Conclusion：ox-LDL can induce the expression of VSMC ACE mRNA in which LOX-1 plays a vital rule.