摘要
Formaldehyde,a well-known environmental toxic hazard,has been found to produce endogenously via semicarbazide-sensitive amine oxidase-catalyzed oxidative deamination of methylamine.In diabetes,the activity of SSAO has been found to increase with a subsequent increase in endogenous formalde-hyde production.It has been postulated that SSAO-induced production of formaldehyde may be in-volved in the alteration of protein structure,which may subsequently cause protein deposition associ-ated with chronic pathological disorders.Formaldehyde has also been found to react(cross-link) with amino group of the N-terminal amino acid residue and with the side-chains of arginine,cysteine,his-tidine and lysine residues.Therefore,formaldehyde may be responsible,at least in part,for protein cross-linkage,oxidative stress and cytotoxicity.The cross-linking of formaldehyde with bovine serum albumin was studied using LC-MS and Mascot database.The peptides sequence for control BSA(un-treated) digested with trypsin was matched in the online database search query by exporting the MS/MS data to online MASCOT database.In this way,a total of twenty-seven peptides were matched in the database search query.These twenty-seven peptides were then searched manually in all of the tryptic BSA samples treated with different concentrations of FA that were incubated in different time intervals.Six formaldehyde-treated BSA peptides(FKDLGEEHFK,HLVDEPQNLIK,KVPQVSTPTLVEVSR,RPCFSALTPDETYVPK,LVNELTEFAK,DAFLGSFLYEYSR) were found to be the possible markers for formaldehyde-protein/peptides adducts.
Formaldehyde, a well-known environmental toxic hazard, has been found to produce endogenously via semicarbazide-sensitive amine oxidase-catalyzed oxidative deamination of methylamine. In diabetes, the activity of SSAO has been found to increase with a subsequent increase in endogenous formaldehyde production. It has been postulated that SSAO-induced production of formaldehyde may be involved in the alteration of protein structure, which may subsequently cause protein deposition associated with chronic pathological disorders. Formaldehyde has also been found to react （cross-link） with amino group of the N-terminal amino acid residue and with the side-chains of arginine, cysteine, histidine and lysine residues. Therefore, formaldehyde may be responsible, at least in part, for protein cross-linkage, oxidative stress and cytotoxicity. The cross-linking of formaldehyde with bovine serum albumin was studied using LC-MS and Mascot database. The peptides sequence for control BSA （untreated） digested with trypsin was matched in the online database search query by exporting the MS/MS data to online MASCOT database. In this way, a total of twenty-seven peptides were matched in the database search query. These twenty-seven peptides were then searched manually in all of the tryptic BSA samples treated with different concentrations of FA that were incubated in different time intervals. Six formaldehyde-treated BSA peptides （FKDLGEEHFK, HLVDEPQNLIK, KVPQVSTPTLVEVSR, RPCFSALTPDETYVPK, LVNELTEFAK, DAFLGSFLYEYSR） were found to be the possible markers for formaldehyde-protein/peptides adducts.
基金
the Nation Natural Science Foundation Key Project of China (Grant No.20435020)
the "11th Five-Year Plan" National Key Technology R&D Program of China (Grant No.2006BAK03A07)
关键词
甲醛
牛
血清白蛋白
时标
生物标志物
formaldehyde （FA）, bovine serum albumin （BSA）, peptides, markers, LC-MS
