P2X3受体在神经源性膀胱大鼠背根神经节及逼尿肌中的表达

P2X3 receptor expression in DRG and bladder detrusor of an animal model of neurogenic bladder and urethra

目的 探讨P2X3受体在不同平面脊髓损伤后神经源性膀胱大鼠L6-S1背根神经节（DRG）和膀胱逼尿肌中的表达变化及临床意义。方法共选取健康雌性SD大鼠80只，将其随机分为骶上脊髓（胸腰段）损伤组、骶髓损伤组及对照组。采用脊髓横断法制备大鼠不同平面脊髓损伤后神经源性膀胱模型，于模型制作20d后采用Western blotting技术检测各组大鼠背根神经节和膀胱逼尿肌中P2X3受体表达情况，并进行组间比较。结果骶上脊髓损伤组大鼠背根神经节、膀胱逼尿肌中P2X3受体表达水平较对照组、骶髓损伤组明显增高（P〈0．01）；骶髓损伤组背根神经节、膀胱逼尿肌中P2X3受体表达水平较对照组明显降低（P〈0．05）。结论结合本课题前期研究的尿流动力学检查结果，推测P2X3受体表达水平与膀胱尿道功能障碍程度密切相关，有望通过影响P2X3受体表达水平为临床治疗脊髓损伤后神经源性膀胱提供新途径。

Objective To investigate the P2X3 receptor expression in L6-S1 dorsal root ganglion （DRG） and bladder detrusor in a rat model of neurogenic bladder and urethra. Methods Eighty Sprague-Dawley rats were recruited and randomly divided into a sacral injury group, a suprasaeral injury group and a control group. Spinal transection was performed to establish the animal model of neurogenic bladder and urethra in rats of the sacral injury group and suprasacral injury group. Check the P2X3 receptor expression in DRG and bladder detrusor among the three groups by Western blot test at 20 days after model establishment. Results P2X3 receptor expression in L6-S1 DRG of sacral injury group was significantly less than that of the suprasacral injury group, which was in turn significantly higher than that of the control group. P2X3 receptor expression in bladder detrusor of sacral injury group was significantly lower than that of the suprasacral injury group, which was in turn significantly higher than that of the control group. Conclusion There was close relationship between P2X3 receptor expression and dysfunction of bladder and urethra.