摘要
目的:探讨鼻咽癌(NPC)组织中胰岛素样生长因子1受体(IGF-1R)蛋白的表达及其临床意义。方法:应用免疫组织化学方法检测63例NPC组织和鼻咽部炎性黏膜组织中IGF-1R的表达,对其中45例NPC患者进行5年随访观察,以及采用免疫印迹法检测3种体外培养的NPC细胞系中IGF-1R蛋白的表达情况。结果:63例NPC和21例鼻咽黏膜(伴慢性炎症)组织中的IGF-1R阳性表达率分别为82.5%和14.3%,在NPC中的表达阳性率明显高于鼻咽炎性组织(P〈0.01)。NPC组织中IGF-IR的表达与TNM分期和颈淋巴结转移有关,有颈淋巴结转移者其表达阳性率较高(P〈0.05);Ⅰ-Ⅱ期表达阳性率较Ⅲ-Ⅳ期低(P〈0.01);NPC局部复发者IGF-lR阳性表达率(89.5%)明显高于无局部复发者(53.9%),P〈0.05;NPC放疗后生存期小于5年者的阳性表达率(88.9%)明显高于5年以上生存期者的阳性表达率(51.9%),P〈0.01;病理分化程度越高IGF-lR阳性表达率越低,但NPC病理类型间比较差异无统计学意义(P〉0.05)。在3种NPC细胞系中IGF-1R的表达明显高于鼻咽炎症黏膜组织,其中TW03表达最强,CNE2其次,CNEl表达稍弱。结论:NPC组织中存在IGF-1R的过表达现象,IGF-1R的异常表达与NPC的发生、发展存在一定的关系。IGF-1R可能是复发或转移性NPC治疗中一个潜在的靶分子。
Objective:To evaluate the expression of IGF-1R protein and its clinical signification in nasopharyngeal carcinoma.Method:SP immunohistochemical staning was performed to detect the expression of IGF-1R in 63 cases of NPC and 21 inflamatory nasopharyngeal mucosa;and the expression of IGF-1R in three NPC cell lines and chronic nasopharngotis tissues was examined by western blots.Result:The positive rates of IGF-1R were 82.5%and 14.3% respectively in 63 cases of NPC and 21 inflamatory nasopharyngeal mucosa;It also showed stronger positive expression in Stage Ⅲ~Ⅳ of NPC than that in stage Ⅰ~Ⅱ(P〈0.01);IGF-1R positive expression in cases of NPC with cervical lymph node metastasis was significantly higher than that of NPC without lymph node metastasis(P〈0.05);it more often appears in recurrent cases than in primary cases(P〈0.05);IGF-1R positive expression had a significantly stronger in patients whose survival time less than 5 years than that in patients whose survival time over 5 years(P〈0.01).Conclusion:Over-expression of IGF-1R is related to poor differentiation of NPC,also may be linked with oncogenesis and development of NPC.The over-expression of IGF-1R may be one of a prognostic factors in NPC.The IGF-1R might be a potential therapeutic target in nasopharyngeal cancer.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2008年第19期874-877,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金
湖北省卫生厅资助重点项目(No:WJ301140502)
关键词
鼻咽肿瘤
胰岛素样生长因子1受体
免疫组织化学
免疫印迹法
Nasopharyngeal carcinoma
Insuline like growth factor-1 receptor
Immunohistochemistry
Western blots