胰岛素样生长因子-1受体蛋白在鼻咽癌细胞中的表达及其临床意义

Expression and clinical significance of insuline like growth factor-1 protein in nasopharyngeal carcinoma

目的：探讨鼻咽癌（NPC）组织中胰岛素样生长因子1受体（IGF-1R）蛋白的表达及其临床意义。方法：应用免疫组织化学方法检测63例NPC组织和鼻咽部炎性黏膜组织中IGF-1R的表达,对其中45例NPC患者进行5年随访观察,以及采用免疫印迹法检测3种体外培养的NPC细胞系中IGF-1R蛋白的表达情况。结果：63例NPC和21例鼻咽黏膜（伴慢性炎症）组织中的IGF-1R阳性表达率分别为82.5%和14.3%,在NPC中的表达阳性率明显高于鼻咽炎性组织（P〈0.01）。NPC组织中IGF-IR的表达与TNM分期和颈淋巴结转移有关,有颈淋巴结转移者其表达阳性率较高（P〈0.05）;Ⅰ-Ⅱ期表达阳性率较Ⅲ-Ⅳ期低（P〈0.01）;NPC局部复发者IGF-lR阳性表达率（89.5%）明显高于无局部复发者（53.9%）,P〈0.05;NPC放疗后生存期小于5年者的阳性表达率（88.9%）明显高于5年以上生存期者的阳性表达率（51.9%）,P〈0.01;病理分化程度越高IGF-lR阳性表达率越低,但NPC病理类型间比较差异无统计学意义（P〉0.05）。在3种NPC细胞系中IGF-1R的表达明显高于鼻咽炎症黏膜组织,其中TW03表达最强,CNE2其次,CNEl表达稍弱。结论：NPC组织中存在IGF-1R的过表达现象,IGF-1R的异常表达与NPC的发生、发展存在一定的关系。IGF-1R可能是复发或转移性NPC治疗中一个潜在的靶分子。

Objective：To evaluate the expression of IGF-1R protein and its clinical signification in nasopharyngeal carcinoma.Method：SP immunohistochemical staning was performed to detect the expression of IGF-1R in 63 cases of NPC and 21 inflamatory nasopharyngeal mucosa;and the expression of IGF-1R in three NPC cell lines and chronic nasopharngotis tissues was examined by western blots.Result：The positive rates of IGF-1R were 82.5%and 14.3% respectively in 63 cases of NPC and 21 inflamatory nasopharyngeal mucosa;It also showed stronger positive expression in Stage Ⅲ～Ⅳ of NPC than that in stage Ⅰ～Ⅱ（P〈0.01）;IGF-1R positive expression in cases of NPC with cervical lymph node metastasis was significantly higher than that of NPC without lymph node metastasis（P〈0.05）;it more often appears in recurrent cases than in primary cases（P〈0.05）;IGF-1R positive expression had a significantly stronger in patients whose survival time less than 5 years than that in patients whose survival time over 5 years（P〈0.01）.Conclusion：Over-expression of IGF-1R is related to poor differentiation of NPC,also may be linked with oncogenesis and development of NPC.The over-expression of IGF-1R may be one of a prognostic factors in NPC.The IGF-1R might be a potential therapeutic target in nasopharyngeal cancer.