静脉输注内皮祖细胞治疗大鼠急性心肌梗死的实验研究

Intravenous Transfusion of Endothelial Progenitor Cells in Rats with Acute Myocardial Infarction

目的:探讨静脉输注体外培养扩增的内皮祖细胞(EPC)对急性心肌梗死大鼠的血管新生、心肌梗死面积以及心功能的影响。方法:分离大鼠骨髓单个核细胞,EBM-2+Single Quots培养基培养,鉴定EPC表面标志,EGFP-Ad标记。建立大鼠急性心肌梗死模型,在心梗后24 h,将体外培养的EPC经尾静脉缓慢注入动物体内。分别在细胞输注后7,14,28 d,检测输注细胞在心肌梗死周边区的分布、新生血管密度、心梗面积以及心功能等多项指标。结果:细胞输注后第7天,EPC治疗组心梗面积(25.08±1.18)%,明显低于对照组的(32.63±1.16)%;细胞输注后第14天时,EPC治疗组梗死周边区可见大量EGFP阳性细胞,并形成管腔样结构,EPC治疗组血管密度为(14.36±1.48)/mm2,明显高于对照组的(6.33±0.69)/mm2;细胞输注第28天,EPC治疗组大鼠各项心功能指标均明显优于对照组。结论:静脉输注的EPC能有效的向心肌梗死周边区募集,形成新生血管,减少心肌梗死面积,促进心功能的恢复。

Objective： To investigate the effect of endothelial progenitor cells （EPCs） on neovascularization, in farct area and cardiac function in rat acute myocardial infarction model. Methods： Rat bone marrow-derived mononuclear cells （MNCs） were cultured in endothelial basal medium. The cell markers were assayed by immunocytochemistry. The left anterior coronary artery was ligated to establish the myocardial infarction model. The EPCs or blank medium were transfused into the circulation by tail vein 24 hours after the ligation. After 7, 14 and 30 days, infarction area, vessel density and cardiac function were measured. Results. At 7 days after tansfusion, the percentage of infarction area in EPC group was （25. 08±1.18）%, which was lower than that in control group （32. 63± 1.16）（P〈0.01）. After 14 days, the vessel density was distinctly increased in EPCs group. The labelled EPCs could be detected in the infracted border zone. The cardiac function was improved in the EPCs group. Conclusion. Intravenous transfusion of EPCs can effectively recruit EPCs into the infracted border zone, promote neovascularization, reduce infarct area and improve cardiac function.