葡萄糖对丁酸钠诱导的结肠癌细胞增殖抑制和凋亡效应的调节作用及其可能机制

Effects of Glucose on Proliferation Inhibition and Apoptosis of Colon Cancer Cells Induced by Sodium Butyrate

目的:探讨葡萄糖对丁酸钠诱导的结肠癌细胞增殖抑制和凋亡效应的影响,并探讨其可能机制。方法:用MTT法检测细胞存活率。细胞凋亡用TUNEL法检测。用RT-PCR法检测葡萄糖转运蛋白1(GLUT1)、单羧酸载体1(MCT1)的mRNA表达水平。结果:低浓度葡萄糖诱导HT-29细胞系凋亡、调节其增殖,且葡萄糖能够明显抵制丁酸钠诱导凋亡和增殖抑制作用,同时对GLUT1 mRNA、MCT1 mRNA也有一定抑制作用。结论:葡萄糖浓度变化能够明显影响丁酸钠的诱导凋亡和增殖抑制作用,这种影响可能与细胞内葡萄糖和丁酸钠的浓度有关。

Objective： To learn about the influence of glucose on proliferation inhibition and apoptosis of colon cancer cells induced by sodium butyrate, and explore its possible mechanism. Methods： Cell survival rates were detected by MTT assay. Apoptosis was detected by TUNEL assay. Glucose transport protein 1 （GLUT1） and monoearboxylate transporter 1 （MCT1） mRNA expression was detected by RT-PCR. Results： Low concentration of glucose induced apoptosis and regulated pro- liferation in HT-29 cell line, and glucose can obviously inhibit the effect of proliferation inhibition and apoptosis induced by sodium butyrate. Glucose also down-regulated the expression of MCT1 mRNA and GLUT1 mRNA. Conclusion： Glucose can regulate the effect of proliferation inhibition and apoptosis of colon cancer cells induced by sodium butyrate and this influence may be associated with the intracellular concentration of glucose and sodium butyrate.