霉酚酸对白蛋白刺激肾小管上皮细胞表达趋化因子的影响

Effects of Mycophenolic Acid on the Expression of Chemokines of Tubular Cells Induced by Albumin in Vitro

目的:探讨霉酚酸对白蛋白刺激肾小管上皮细胞趋化因子RANTES表达的影响。方法:培养大鼠肾小管上皮细胞(NRK-52E),分别加入白蛋白或/和霉酚酸。应用RT-PCR方法测定RANTES mRNA表达,应用Western blot测定RANTES蛋白的表达。结果:白蛋白呈时间依赖性上调肾小管上皮细胞RANTES mRNA和蛋白表达增加。霉酚酸可抑制肾小管上皮细胞RANTES的表达。结论:霉酚酸可抑制白蛋白引起的肾小管上皮细胞分泌RANTES而发挥其抗炎作用。

Objective： To investigate the effect of mycophenolic acid （MPA） on the expression of chemokines RANTES in renal tubular epithelial cells stimulated by albumin. Methods： The renal tubular epithelial NRK-52E cells were incubated in the presence of albumin （30 g/L） with or without MPA. Expression of mRNA was analyzed by RT-PCR. Expression of protein RANTES was assessed by Western blot. Results： Albumin stimulated expression of RANTES mRNA and protein in a time-dependent manner. MPA inhibited albumin-induced the expression of RANTES. Conclusion： MPA may inhibit the expression of RANTES in renal tubular epithelial cells.