摘要
目的:从广西产的刺楸茎皮中提取分离具有降血糖活性的有效部位或有效成分。方法:经醇提、系统分离、硅胶和大孔树脂分离获得3个部分的提取物(A、B、C),分别进行降血糖药效试验,确定有效部位,并对有效部位再进行分离和单体成分的鉴定。结果:在有效部分提取物(A)中获得3个单体成分,分别为丁香脂苷[lirioden-drin(Ⅰ)],刺楸皂苷B(Ⅱ)和刺楸皂苷H(Ⅲ);经相应分离和药效试验证实,具有降血糖作用的部位存在于提取物(A)中,而在提取物(B)和提取物(C)未见明显的降血糖作用。结论:首次在刺楸中发现丁香脂苷成分,并且提取物(A)经药效实验证实是具有降血糖作用的有效部位。
Objective: To study the hypoglycemic active fractions or constituents that were extracted and isolated from the stem bark of Kalopanax septemlobus (Thunb.) Koidz. in Guangxi. Methods: Three fractions were obtained by extracting with alcohol, separating systematically and isolating by the silica and macroporous resin chromatography methods. The hypoglycemic activity of the constituents from the three fractions respectively was evaluated using diabetic models induced by alloxanin in mice. Then the effective fraction was separated by pharmacodynamic test and the monomer components were identified from the effective fraction. Results: Three monomer components, liriodendrin(Ⅰ), Kalopanax saponine B(Ⅱ ) and Kalopanax saponine H(Ⅲ), were obtained from the only effective fraction of all the three fractions. Conclusion: Liriodendrin( Ⅰ ) is thought to be found firstly in this plant, and the fraction extracted from Kalopanax septemlobus (Thunb.) Koidz. in Guangxi showes an excellent hypoglycemic activity.
出处
《武汉大学学报(医学版)》
CAS
2008年第6期759-762,共4页
Medical Journal of Wuhan University
基金
广西自然科学基金资助项目[桂科计字(2004)47号]
关键词
刺楸
刺楸皂苷
化学成分
丁香脂苷
降血糖
Kalopanax septemlobus (Thunb.)Koidz.
Kalopanax Saponine
Chemical Composition
Liriodendrin
H ypoglycemic