摘要
目的:建立结肠炎定位片中大黄素、大黄酚及大黄酸的含量测定方法。方法:采用HPLC法,使用KromasilC18柱,流动相为甲醇-0.1%磷酸(88∶12),紫外检测波长为254 nm,柱温为36℃。结果:此方法线性关系良好且能使大黄素、大黄酚及大黄酸3种成分很好地分离,加样回收率分别为大黄素100.3%,峰面积积分值(RSD)1.39%;大黄酚99.5%,RSD 1.62%;大黄酸100%,RSD 0.73%。结论:本方法简便快速,结果准确,可用于控制结肠炎片的质量。
Objective: To develop a method to determine rhien, emodin and chrysophanol contents in colon site-specific drug delivery tablets. Methods: HPLC method was used. The separation was performed on SYMMETRY column with methanol-0. 1% phosphate acid (88 : 12) as a mobile phase. Results: The resolution and the linearity of the method were good. The average recovery rate was 100.3% and RSD was 1.39% for emodin, and the two indices was 99.5% and 1.62% respectively for chrysophanol, 100% and 0.73% for rhiern. Conclusion: The method is simple, rapid and reliable. It is suitable for quality control of colon site-specific drug delivery tablets.
出处
《武汉大学学报(医学版)》
CAS
2008年第6期772-774,共3页
Medical Journal of Wuhan University
基金
湖北省教育厅重点自然科学项目(编号:D200662001)
荆州市科技局自然科学项目(编号:200217E07-15)
关键词
结肠炎定位片
大黄素
大黄酚
大黄酸
HPLC
含量
Colon Site-Specific Drug Delivery Tablets
Rhiern
Emodin
Chrysophanol
HPLC
Contents