HPLC法测定结肠炎定位片中大黄素、大黄酚及大黄酸的含量

Determination of Rhiern,Emodin and Chrysophanol in Colon Site-Specific Drug Delivery Tablets by HPLC

目的:建立结肠炎定位片中大黄素、大黄酚及大黄酸的含量测定方法。方法:采用HPLC法,使用KromasilC18柱,流动相为甲醇-0.1%磷酸(88∶12),紫外检测波长为254 nm,柱温为36℃。结果:此方法线性关系良好且能使大黄素、大黄酚及大黄酸3种成分很好地分离,加样回收率分别为大黄素100.3%,峰面积积分值(RSD)1.39%;大黄酚99.5%,RSD 1.62%;大黄酸100%,RSD 0.73%。结论:本方法简便快速,结果准确,可用于控制结肠炎片的质量。

Objective： To develop a method to determine rhien, emodin and chrysophanol contents in colon site-specific drug delivery tablets. Methods： HPLC method was used. The separation was performed on SYMMETRY column with methanol-0. 1% phosphate acid （88 ： 12） as a mobile phase. Results： The resolution and the linearity of the method were good. The average recovery rate was 100.3% and RSD was 1.39% for emodin, and the two indices was 99.5% and 1.62% respectively for chrysophanol, 100% and 0.73% for rhiern. Conclusion： The method is simple, rapid and reliable. It is suitable for quality control of colon site-specific drug delivery tablets.