牦牛胸小骨中硫酸软骨素的提取研究

Extraction of chondroitin sulfate from yak breastbone

以牦牛胸小骨为原料,采用稀碱-酶解法提取硫酸软骨素,对碱解条件和酶解过程进行筛选.碱提取的最佳条件为：原料与1.5%的NaOH液质量比1∶1.2,38℃搅拌提取10 h;酶水解最佳条件为：胰酶体积分数8%,pH8.5～9.0,48～51℃水解5～6 h.在最佳提取条件下,得到牦牛胸小骨硫酸软骨素产品为白色粉末,纯度92.5%,收率为2.67%.

hondroitin sulfate （CS） was extracted from yak breastbone by using sparse alkali-enzymatic decompounding method. Some technique conditions of alkali extracting and enzymatic decompounding were screened by orthogonal experiment. The results showed that the optimum condition for alkali extracting was 1.5% NaOH as extraction solvent, the ratio of material to solvent 1 ： 1.2, controlling temperature at 38 ℃ and stirring for 10 hours, The optimum condition for enzymatic decompounding was trypsinogen 8 %,temperature 48-51 ℃, pH 8.5 - 9.0 and reaction time 5-6 hours. Under above extracting conditions,CS was obtained as white powder,with 92.5 % pure degree and 2.67% of extracting rate.