P27^(kipl)和ki-67蛋白在病理性瘢痕组织中的表达和意义

Expression and significance of P27^(kipl) and ki-67 protein in pathological scar

目的:研究病理性瘢痕组织中P27kipl和ki-67蛋白的表达情况及其相互关系,探讨他们在瘢痕形成中的作用及机制。方法:应用免疫组化SP法检测正常皮肤、成熟瘢痕、增生性瘢痕和瘢痕疙瘩组织中P27kipl、ki-67蛋白的表达并进行统计学分析。结果:病理性瘢痕组织中P27kipl蛋白表达显著低于正常皮肤、成熟瘢痕(P=0.036);ki-67蛋白在病理性瘢痕组织中的表达显著高于正常皮肤、成熟瘢痕(P<0.000);病理性瘢痕组织中P27kipl的表达与ki-67蛋白表达呈负相关(P<0.000)。结论:P27kipl蛋白表达的下降及ki-67的表达升高可能与病理性瘢痕的形成有关。

Objective To study the expression of P27kjpl and ki-67 in the pathological scars and their reaction-ship and to investigate its role and its possible mechanism in the pathogenesis of abnormal scars. Methods Immunohistochemiscal technique was performered to detect the expression and distribution of P27kjpl and ki-67 protein in hypertrophic scar （42cases）, keloid （18cases）, normal scar （40 cases） and normal skin （50cases）,statistics was used to analyze the data. Results The positive rate of P27kjpl and ki-67 protein expression were remarkably significant in comparison between normal scar and abnormal scar （P〈0.05）. In pathological scar the protein of P27kjpl and ki-67 showed a strong inverse correlation （P〈0.000）. Conclusion Low expression of P27kjpl and high expression of ki-67 may play an important role in the proliferation of fibroblasts and in the pathogenesis of pathological scar.