AGE及葡萄糖对THP-1巨噬细胞CXCL16 mRNA表达的影响

Effect of AGE and glucose on CXCL16 mRNA expression in THP-1 macrophages

目的:探讨晚期糖基化终末产物(advanced glycation end products,AGE)及葡萄糖对THP-1巨噬细胞CXCL16 mRNA表达的影响.方法:①选择合适的培养时间;②不同浓度的AGE与THP-1巨噬细胞共同孵育;③不同浓度葡萄糖与THP-1巨噬细胞共同孵育;分别用AGE、葡萄糖及同时加入AGE和葡萄糖与THP-1巨噬细胞孵育;⑤采用RT-PCR检测孵育后CXCL16及CD36 mRNA的表达量,观察和比较各组孵育后CXCL16及CD36 mRNA的表达情况.结果:①随着培养液中AGE浓度的增加CXCL16及CD36 mRNA的表达增加;②随培养液中葡萄糖浓度的增加CXCL16及CD36 mRNA的表达增加;③AGE及葡萄糖同时作用于THP-1巨噬细胞时,对CXCL16及CD36 mRNA的表达上调作用大于AGE或葡萄糖单一因素的影响.结论:AGE及葡萄糖呈时间和浓度依赖性上调THP-1巨噬细胞CXCL16 mRNA的表达,并且AGE及葡萄糖两者合用可以进一步增加这种表达.这可能为糖尿病动脉粥样硬化机制的探讨提供新的线索.

AIM ： To investigate the effect of advanced glycation end products（AGE） and glucose on CXCL16 mRNA expression in THP- 1 macrophages. METHODS： （1）An appropriate incubation time was determined; （2）THP-1 macrophages were treated with AGE at different concentrations; （3）THP-1 maerophages were treated with glucose at different concentrations; （4）THP-1 macrophages were treated with AGE and glucose respectively and the mixture of AGE and glucose; （5）CXCL16 and CD36 mRNA were determined by the reverse transcriptionpolymerase chain reaction （RT-PCR）. The level of expression of CXCL16 and CD36 mRNA was compared within the different treatment groups. RESULTS： （1）The expression of CXCL16 and CD36 mRNA was up-regulated with the concentration of AGE increased; （2）The expression of CXCL16 and CD36 mRNA was also up-regulated with the concentration of glucose increasd ; （3） When THP-1 macrophages were treated with the mixture of AGE and glucose, the expression of CXCL16 and CD36 mRNA was higher than that of ones treated with AGE or glucose alone. CONCLUSION： The AGE and glucose can up-regulate the expression of CXCL16 mRNA in a timedependent and dose-dependent manner in THP-1 macrophages, which may provide a new clue for the investigation of the mechanisms of diabetic atherosclerosis.