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RERG钾通道在大鼠肺泡巨噬细胞中的表达

Expression of RERG potassium channel in rat alveolar macrophages
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摘要 目的:观察RERG钾通道在大鼠肺泡巨噬细胞中有无表达.方法:以大鼠肺泡巨噬细胞系NR8383为研究对象,RT-PCR方法检测NR8383细胞膜rerg钾通道mRNA,并测序验证;免疫细胞化学方法检测NR8383细胞膜上RERG钾通道蛋白.结果:NR8383细胞中可以检测到rerg钾通道mR-NA,并经测序证实;NR8383细胞膜上可以检测到RERG蛋白.结论:从mRNA和蛋白水平证明RERG钾通道在大鼠肺泡巨噬细胞上有表达,为进一步探索RERG钾通道与肺泡巨噬细胞功能的关系提供了依据. AIM： To investigate whether RERG potassium channels are expressed in rat alveolar macrophages. METHODS： The expression of rerg mRNA in NR8383 ceils, a rat alveolar macrophage cell line, was investigated by reverse transcription polymerase chain reaction （RT-PCR） and verified by sequencing. The expression of RERG protein was investigated by immunohistochemistry. RESULTS ： The mRNA and protein of RERG potassium channels were found in NR8383 cells. CONCLUSION： The mRNA and protein of RERG potassium channels are expressed in rat alveolar macrophages, which supplys a theoretical basis for exploring the relationship between RERG potassium channel and function of alveolar maerophage.
作者 董海莹 董明清 李志超 王晓斌 柳君泽
机构地区 第三军医大学高原医学系病理生理学教研室 第四军医大学病理与病理生理学教研室
出处 《第四军医大学学报》 北大核心 2008年第20期1900-1902,共3页 Journal of the Fourth Military Medical University
基金 国家自然科学基金(30700265 30770925)
关键词 巨噬细胞 肺泡 RERG钾通道 NR8383 大鼠 macrophages, alveolar RERG potassium channel NR8383 rat
分类号 R363 [医药卫生—病理学]
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参考文献10

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第四军医大学学报
2008年 第20期

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