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MiR-181c对人脑胶质瘤细胞增殖及凋亡的作用 被引量：1

Effects of miR-181c on cell proliferation and apoptosis of human brain glioma cells
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摘要 目的观察miR-181c对人脑胶质瘤细胞增殖及凋亡的影响,进一步探讨miR-181c的生物学功能。方法化学合成miR-181c,脂质体转染U251细胞。应用cck-8法和流式细胞术检测细胞的增殖和凋亡。结果miR-181c上调后对U251细胞具有抑制作用,且呈明显的剂量依赖性,而对照组无明显抑制作用。结论化学合成的miR-181c在人脑胶质瘤细胞增殖和凋亡过程中发挥重要作用,可能成为胶质瘤基因治疗的新靶点。 Objective To explore the biological function of miR--181c, and observe the effects of miR--181c on cell proliferation and apoptosis of human brain glioma cells. Methods MiR--181c was synthesized and transfected into the U251 malignant glioma cells by liposome. Cell viability was assessed by cck--8 assay. Cell apoptosis was detected by flow cytometry. Results MiR--181c inhibited U251 cell proliferation in a dose dependent manner, while the control group had no obvious effect on cell growth. Conclusions The chemically synthesized miR--181c plays an important role in cell proliferation and apoptosis of human brain glioma cells, which may be a promising target for glioma gene therapy.
作者 刘书芳 胡锦 董万利 柏燕燕 金由辛
机构地区 苏州大学附属第一医院神经内科 上海交通大学附属第六人民医院神经外科 中国科学院生化所分子生物学国家重点实验室
出处 《神经疾病与精神卫生》 2008年第5期335-337,共3页 Journal of Neuroscience and Mental Health
基金 国家自然科学基金(编号:30371459) 上海市科学技术发展基金(编号:034047)资助
关键词 微RNAMiR-181c 胶质瘤 细胞凋亡 MicroRNA MiR--181c Glioma Apoptosis
分类号 R739.4 [医药卫生—肿瘤]
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参考文献11

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同被引文献36

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神经疾病与精神卫生
2008年 第5期

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