Survivin基因在脑星形细胞肿瘤中的表达及其病理意义

The Expression and Pathological Significance of Survivin mRNA in Astrocytomas

目的:研究脑星形细胞肿瘤中Survivin基因mRNA表达的临床、病理和预后的关系。方法:应用RT-PCR方法检测26例脑星形细胞肿瘤组织中Survivin基因mRNA的表达,并通过电泳凝胶图像系统检测Survivin的相对表达量。结果:Survivin只表达于脑星形细胞肿瘤中的肿瘤细胞,对正常脑组织中胶质细胞无表达。在脑星形细胞肿瘤中RT-PCR的检出率为65%(17/26)。Survivin基因mRNA的相对表达量与脑星形细胞肿瘤的病理分级密切相关,在高级别肿瘤中Survivin基因mRNA相对表达量和表达强度均高于低级别肿瘤(P<0.05)。结论:随着星形细胞肿瘤分化程度的降低,Survivin相对表达量显著增强;其高表达预示着预后不良,可成为有价值的评估该肿瘤预后指标。

Objective：To investigate the relationships between the expression of Survivin gene （at mRNA levels）and the clinico- pathologic parameters in astroeytomas ,and evaluate its significance in predicting the prognosis.Methods：The expression of Survivin mRNA in 26 fresh astrocytomas was examined by RT-PCR. The relative expression of Survivin by electrophoresis gel image system was calculated. Results：The expression of Survivin was only found in tumor eells in astroeytomas and there was no expression in normal brain tissues. The positive rate of RT-PCR was 65%（17/26）.The relative expression of Survivin by RT-PCR had a high correlation with pathological grades of astrocytomas. The relative expression and expression intensity of Survivin gene in high-grade tumors were higher than those in low-grade tumors （P〈0.05）.Conclusion ：The relative expression and expression intensity of Survivin gene reinforced sig- nificantly when the differentiation degree decreased. Survivin might play an important role in astroeytomas conversion process from a low degree to a higher degree. Survivin can become a valuable evaluation marker of prognosis.