全氟辛烷磺酸对雌鹌鹑的生殖毒性研究

Study on Reproductive Toxicity of Perfluorooctane Sulfonate(PFOS)in Female Quail

为了探讨全氟辛烷磺酸(PFOS)对雌性禽类的生殖毒性,以雌鹌鹑为禽类指示动物进行了PFOS经口染毒实验.染毒组PFOS染毒剂量分别为12.5、25.0、50.0mg·kg-1(以饲料中的PFOS计),同时设对照组,连续染毒71天,检测体重、产卵率、受精率、孵化率及雏鹌鹑死亡率、畸形率等指标.结果表明,染毒第33天起50.0mg·kg-1剂量组雌鹌鹑体重显著低于对照组(p<0.01),并出现死亡;各染毒组开始产卵时间均滞后于对照组,产卵率均低于对照组;各染毒组雌鹌鹑受精率、孵化率、孵化12h后雏鹌鹑体重均低于对照组,雏鹌鹑畸形率、孵化后12h内雏鹌鹑死亡率均高于对照组;随着染毒剂量的增加,雌鹌鹑血清及卵黄中PFOS含量也相应升高,均呈显著正相关(p<0.01);各染毒组雌鹌鹑开始产卵5天内所产卵中PFOS含量均显著高于实验结束前3天所产卵(p<0.01).以上研究结果提示,PFOS可推迟雌鹌鹑产卵时间,降低产卵率,对受精率、孵化率及孵化后雏鹌鹑的生存和发育均有不利影响.

To approach the reproductive toxicity of perfluorooctane sulfonate（PFOS, C8F17SO3-） in female avians, an oral administration experiment was performed in the female quail that can be applied as animal indicators. The dose of PFOS were 0, 12.5, 25.0, 50.0mg·kg^-1（calculated with PFOS in forage）, respectively. The quail were administrated with PFOS for 71 days when body weight, egg production rate, hatchability, mortality of poults, and deformity rate were evaluated. Results indicated that the body weights of female quail in 50.0mg·kg^-1 dosage group were significantly lower than those in control group after 33 days exposure to PFOS（p〈0.01）, and mortality was also observed. There was a hysteresis in the beginning time of egg laying in dose groups, and the overall egg laying rates of female quail were significantly lower than those in control group. The rates of fertilization, hatchabilities and body weights of poults in 12 hours after hatching in dose groups were lower than those in control group. Moreover, the deformity rates and mortalities of poults in 12 hours after hatching in dose groups were higher than those in control group. The concentrations of PFOS in serum and egg yolk rose with the increase of PFOS dose. The levels of PFOS in the eggs that laid within 5 days after beginning of egg laying were significantly higher（p〈0.01） than those laid within 3 days before the experiment termination, and significant positive correlation was observed（p〈0.01）. Results of the study suggested that PFOS postponed the beginning time of egg laying in female quail, and had adverse effects on the fertilization rate, hatchability of female quail, as well as survival and development of poults after hatching.