黄芪总皂苷合三七总皂苷对实验性脑缺血再灌注脑水肿和脂质过氧化的抑制作用

Effect of Astragalus and Pnanx Notoginsong on Cerebral Edema and Lipid Peroxidation of Cerebral Ⅱschemia Reperfusion of Animols

目的:观察黄芪总皂苷合三七总皂苷(TSA+PNS)对实验性脑缺血再灌注脑水肿和脂质过氧化的影响。方法:结扎大鼠的双侧颈总动脉,复制脑缺血再灌注模型,静脉注射给药,观察TSA+PNS对再灌注后脑组织含水量、脑血管对伊文思蓝通透性的影响。结扎小鼠的双侧颈总动脉,复制脑缺血再灌注模型,静脉注射给药,观察TSA+PNS对手术后12、18、24h脑组织SOD活力、CAT活力和MDA浓度的影响。结果:和假手术组比较,模型动物脑组织含水量、伊文思蓝含量和MDA浓度明显升高(P<0.01),SOD活力、CAT活力明显降低(P<0.01);应用TSA+PNS后,可提高SOD活力、CAT活力,降低MDA浓度、脑组织含水量、伊文思蓝含量(P<0.01～0.05)。结论:TSA+PNS对脑缺血再灌注导致的脑组织水肿、脑血管的通透性和脂质过氧化反应有抑制作用,提高SOD活力、CAT活力可能是其重要的作用机制。

Objective： To investigate the effect of total saponins of Astragalus（TSA） and total saponins of Pnanx notoginseng（PNS） on cerebral edema and lipid peroxidation in cerebral ischemia reperfusion of animals. Methods： Two common carotid arteries of rats or mice were ligated to make the model of cerebral ischemia reperfusion. Then drugs were given by vein. Many indexes were detected, including of water content of cerebrum, content of EB and MDA, activity of SOD, CAT in cerebral tissue. Results ：Compared with vehicle group, CAT and SOD activities were increased significantly and MDA content, water content of cerebrum,content of EB were decreased significantly in TSA and PNS group （ P 〈 0.05 - 0.01 ）. Conclusion： TSA and PNS could inhibit cerebral edema, cerebral edema lipid peroxidation of cerebral ischemia reperfusion, and increase activities of CAT and SOD.