摘要
O^6-甲基脱氧鸟嘌呤核苷一磷酸由O^6-甲基脱氧鸟嘌岭苷与磷酸三甲酯在-10℃反应得粗品,再经 DEAE-Sephadex 和 Tsk-gel Toyopearl 二次柱层析纯化,最后用甲醇、丙酮和乙醚(1:1:8)重结晶,得无色针状结晶。
O^6-Methyideoxyguanosine monophospate wasobtained by the reaction between trimethyl phosphate and O^6-methyldeoxyguanosine in arthydrou,tricthylamine at—10℃.It crystallized as colour-less long needies from methanol,acetone andethylether (1:.UV-absorption sptra λ_(ma).287nm,245nm(pH1);281nm,249nm(pH7);280nm、250nm(pH11).HPLC(ODS 5μm 0.46×15cm),8min(eluant 0.05 mol KH_2PO_4+8%MeOH_ pH4.0).Chromatograghy on DEAE Paper,R_0.91(0.25mol NHHCO_3).Thin layer chromatgraphy on 300 PEI,R_f 0.73(0.3mol KH_2PO_4 pH4.5).
出处
《化学试剂》
CAS
CSCD
北大核心
1990年第2期112-113,共2页
Chemical Reagents
