茚虫威10%悬浮剂的液相色谱分析方法

A Quantitative Analysis of Indoxacarb 10% Supension Concentrate by HPLC

采用Diamond C18不锈钢柱[150mm×4．6mm（i．d．），5μm]和二极管阵列检测器，以甲醇-水混合溶剂（80：20，v/v）为流动相，流速1．0mL／min、检测波长310nm和柱温35aC，建立了茚虫威制剂10%悬浮剂的高效液相色谱分析方法。茚虫威在0．5-10mg／L的浓度范围内，其峰面积（Y）与质量浓度（x）呈现良好的相关性，其线性回归方程为y=23372x＋3238．2，R2=0．9993。在10％悬浮剂中，茚虫威5个添加浓度水平的平均回收率为99．29％，相对标准偏差（RSD）为1．05％，说明所建立的方法快速、灵敏度高、重现性好，可用于茚虫威10％悬浮剂产品质量控制与分析。

A quantitative high performance liquid chromatography method using diode array detector has been developed for the determination of the active ingredient indoxaearb in the formulation 10% suspension concentrate （SC）. A diamond C18 stainless steel column [150 mm×4.6 mm （i.d.）, 5 μm] was used with mixture of methanol-water （80：20,v/v）as the mobile phase at a flow rate of 1.0 mL/min,the column temperature 35℃ and the ultraviolet detection wavelength 284 nm. There existed good linear correlation between peak area （y） and concentration （x） of indoxacarb in the concentration range of 0.5-10 mg/L with the regression equation y=23 372x ＋3 238.2,R2=0.999 3. The average recovery of the five spiked levels for indoxacarb 10% SC was 99.29% , relative standard deviation 1.05% and coefficient of variation 0.01%,suggesting that the method should be fast,sensitive,reproducible and practical for the quality control and analysis of indoxacarb 10%SC during the manufacture of the product.