摘要
目的研究鬼臼酰肼哌啶氮氧自由基腙（GP1）及其还原物GP1OH，GP1H对血小板功能和MDA等的影响。方法：用TBA法测AA诱导的兔血小板和Fe(2+)－ascorbicacid诱导的大鼠肝组织匀浆MDA生成，用比浊法测AA和Coll诱导的兔血小板聚集，用放免法测TXB2。结果：GP1及其还原物GP1OH，GP1H对AA刺激兔血小板MDA和Fe(2+)－ascorbicacid诱导的大鼠肝组织匀浆MDA生成均有明显的抑制作用，但对AA和胶原诱导的兔血小板聚集无明显影响，也不影响AA诱导的兔血小板TXB2生成。结论：它们对MDA的影响均通过清除自由基而产生。
AIM:To study the effects of GP1 and its reduced derivatives GP1OH,GP1H on the function of platelets and MDA formation.METHODS:Platelet agge\regation induced by collange(Coll) or arachidonic acid(AA) were assayed by turbidimetry,MDA formation induced by arachidonic acid on rabbit platelets or Fe2+ascorbic acid on liver homogenate of rat were assayed by TBA method,TXB2 were measured by radioimmunoassay.RESULTS:GP1 and its reduced derivatives GP1OH,GP1H all couldmarkedly reduce MDA formation of platelets of rabbit induced by AA and that of liver homogenate of rat induced by Fe2+ascorbic acid but could not influence platelet aggregation induced by AA of rabbit.COUCLUSION:The effect of GP1 ,GP1OH,GP1H on MDA formation wee due to scavengerring free radical
出处
《中国药理学通报》
CAS
CSCD
北大核心
1997年第5期420-422,共3页
Chinese Pharmacological Bulletin
基金
甘肃省教委基金
