三唑磷对稻纵卷叶螟诱导的水稻植株挥发物释放的影响

Effects of Triazophos on Volatiles from Rice Plants Infested by Cnaphalocrocis medinalis

研究了施用三唑磷对稻纵卷叶螟诱导的水稻挥发物的影响。利用固相微萃取法收集水稻挥发物,并通过气相色谱-质谱联用仪对挥发物进行分离和鉴定,共收集到36个水稻挥发物组分,其中已定性的14个,以萜类及其氧化物(11个)为主。在已定性的组分中,异喇叭烯、(-)-α-雪松烯、(+)-β-雪松烯、雪松醇等4个组分在水稻上尚未见报道。不同处理间水稻挥发物种类相同,但挥发物组分相对含量存在差异。三唑磷浓度不同导致5种挥发物组分(占总种类数的13.9%)相对含量的变化;而27种组分(占总种类数的75.0%)相对含量因施药天数不同而存在显著差异;农药与水稻品种、害虫密度等生物因子间的互作使32种水稻挥发物组分(占总种类数的88.9%)相对含量显著改变。

The volatiles from rice plants infested by Cnaphalocrocis medinalis were studied under triazophos application. Thirty-six components were collected from headspace of rice plants by solid phase microextraction, 14 of which were identified by gas chromatography coupled with mass spectrometry, and the major components are terpenes and their oxides （11 kinds）. Four of the identified components including isoledene, （-）-α-cedrene, （＋）-β-cedrene and cedrol have not been reported in rice plants. Different triazophos treatments did not result in the emission of new compounds, but the relative proportions （percent of total peak area） among the compounds in the blend were altered. The proportions of 5 components （13.9 %） had significant differences under triazophos treatment at three levels. The proportions of 27 components （75.0%） changed at different days after triazophos treatment. Furthermore, the proportions of 32 components （88.9%） were significantly changed due to the interactions between triazophos and rice varieties, densities of C. medinalis.