含几丁质酶基因的粪产碱菌工程菌(BC2007)的构建

Construction of Chitnianse Gene Targeted Alcaligenes Faecalis (BC2007)

从苏云金杆菌(Bacillus thuringiensis)中扩增出几丁质酶全长基因,通过克隆,测序得到2025 bp的序列。通过再线生物信息学软件,得到该几丁质酶的一级、二级、三级结构。将该基因通过电转化导入原核表达载体pET29 a,筛选到含该几丁质酶基因的BC2007。初步研究表明BC2007在含胶体几丁质的平板上产生了透明圈。

The chitinase gene （2 025 bp） from Bacillus thuringiensis with high chitinase -producting ability was cloned and sequenced. The primary, secondary and tertiary structures of chitinase were predicted using the bioinformatic softwares on line. The chitianse gene was transformed to Vector pET29a by electropotation, the engineered strain named BC2007 with chitinase gene was obtained and kept. The preliminary result showed that the strain BC2007 has an ability to degradate the chitin on the plate.