转移性癌细胞对表皮生长因子和层粘连蛋白的反应及其三磷酸肌醇含量的测定

The amounts of inositol 1,4,5 triphosphate and it response to epidermal growth factor and laminin of carcinoma substrains with high or low metastatic potentials.

为探讨不同转移性癌细胞（ＭＦＣ和ＣＮＥ－２Ｚ）不同克隆细胞株的三磷酸肌醇（ＩＰ３）含量、侵袭能力以及这些细胞对表皮生长因子（ＥＧＦ）和层粘连蛋白（ＬＮ）的反应性。方法利用放射性受体竞争结合法和人工基底膜（ｍａｔｒｉｇｅｌ）侵袭试验法进行了测定。结果显示了低转移性癌细胞比高转移性者的ＩＰ３含量高，对ＥＧＦ的反应性更大，但ＭＦＣ高、低转移性克隆细胞对ＬＮ的反应性差别无显著意义。而ＣＮＥ－２Ｚ高转移细胞比低转移者对ＬＮ的反应性更大。结论说明高、低转移性癌细胞在跨膜信息传递方面存在差别，深入研究其具体机制将有重要意义。

Objective To examine the IP 3 amounts, invasive potentials, and response to LN and EGF of the substrains with high or low metastatic ability from two kinds of carcinoma cell lines (MFC and CNZ 2Z). Method Radio ligand binding assay and matrigel invasive assay. Results The low metastatic substrains from the two lines had higher amounts of IP 3 and stronger response to EGF than their responsive high ones, but high metastatic substrains from CNZ 2Z had stronger response to LN than the low ones while MFC substrains had not significant difference in response to LN. Conclusion There is internal difference in signal transduction between the high and low metastatic cancer cells, and it is significant to study the difference in detail.