人胚黑质细胞移植治疗帕金森病模型猴的行为观察

Behaviour after transplantation of brain cells into monkey models of parkinson′s disease

观察人胚黑质细胞移植治疗帕金森病（ＰＤ）模型猴的病理行为及改善程度。方法应用甲基－苯甲基－四氢吡啶（ＭＰＴＰ）制备偏侧ＰＤ猴模型，将早期人胚黑质细胞立体定向植入ＰＤ猴脑内，连续半年观察动物行为的改善程度，并结合免疫电镜证实移植神经元存活。结果移植治疗后模型猴运动减少、动作缓慢，肌张力增高较移植前明显改善，肢体震颤几乎消失，向右侧自发性旋转行为减轻，阿朴吗啡（ＡＰＯ）诱发的旋转次数明显少于移植前，且可持续一年，免疫电镜可观察到移植酪氨酸羟化酶阳性神经元与宿主脑细胞建立突触联系。结论植入ＰＤ猴脑内的黑质神经元可与宿主神经细胞建立突触联系，且病理症状改善持续时间较长。

Objective To observe the improved degree of pathogenic behaviour in monkey models of Parkinson′s disease after transplantation of substantia nigra cells of human fetus. Method 1 methy 4 pheny1 1,2,3,6 tetrahydropyridine (MPTP) was used to prepare monkey models of hemiparkinson′s disease, ie, early substantia nigra cells of human fetus were stereotaxically transplanted into PD monkey′s behaviour for six months, using immuno electromicroscopy to prove the transplanted survived neuron. Results After transplantation, the model monkey′s motion slow and muscle hypertonia was appparently improved. Limb tremor almost disappeared. Right spontaneous rotation behavior relieved. Rotating frequency caused by APO became less than before transplanytation, and its effect could last a year. Under the immunoelectromicroscope, we observed that transplanted tyrosine hydroxylase positive neuron was connected with the host brain cells in synapse. Conclusion Substantia nigra neuron transplanted into the PD monkey brain can establish synaptic connection with the host nervous cells. Pathogenic symptoms are improved and the effect may remain for a longer time.